TY - JOUR T1 - RNAi screening comes of age: improved techniques and complementary approaches. JF - Nat Rev Mol Cell Biol Y1 - 2014 A1 - Mohr, Stephanie E A1 - Smith, Jennifer A A1 - Shamu, Caroline E A1 - Neumüller, Ralph A A1 - Perrimon, Norbert KW - Animals KW - Gene Regulatory Networks KW - Genetic Testing KW - Humans KW - Inverted Repeat Sequences KW - RNA Interference KW - RNA, Small Interfering AB -

Gene silencing through sequence-specific targeting of mRNAs by RNAi has enabled genome-wide functional screens in cultured cells and in vivo in model organisms. These screens have resulted in the identification of new cellular pathways and potential drug targets. Considerable progress has been made to improve the quality of RNAi screen data through the development of new experimental and bioinformatics approaches. The recent availability of genome-editing strategies, such as the CRISPR (clustered regularly interspaced short palindromic repeats)-Cas9 system, when combined with RNAi, could lead to further improvements in screen data quality and follow-up experiments, thus promoting our understanding of gene function and gene regulatory networks.

VL - 15 IS - 9 U1 - http://www.ncbi.nlm.nih.gov/pubmed/25145850?dopt=Abstract ER -