TY - JOUR T1 - Applications of high-throughput RNA interference screens to problems in cell and developmental biology. JF - Genetics Y1 - 2007 A1 - Perrimon, Norbert A1 - Mathey-Prevot, Bernard KW - Animals KW - developmental biology KW - genome KW - Humans KW - Phenotype KW - RNA Interference AB -

RNA interference (RNAi) in tissue culture cells has emerged as an excellent methodology for identifying gene functions systematically and in an unbiased manner. Here, we describe how RNAi high-throughput screening (HTS) in Drosophila cells are currently being performed and emphasize the strengths and weaknesses of the approach. Further, to demonstrate the versatility of the technology, we provide examples of the various applications of the method to problems in signal transduction and cell and developmental biology. Finally, we discuss emerging technological advances that will extend RNAi-based screening methods.

VL - 175 IS - 1 U1 - http://www.ncbi.nlm.nih.gov/pubmed/17244760?dopt=Abstract ER -