@article {825831, title = {The Transgenic RNAi Project at Harvard Medical School: Resources and Validation.}, journal = {Genetics}, volume = {201}, year = {2015}, month = {2015 Nov}, pages = {843-52}, abstract = {

To facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi Project (TRiP) at Harvard Medical School (HMS) was established along with several goals: developing efficient vectors for RNAi that work in all tissues, generating a genome-scale collection of RNAi stocks with input from the community, distributing the lines as they are generated through existing stock centers, validating as many lines as possible using RT-qPCR and phenotypic analyses, and developing tools and web resources for identifying RNAi lines and retrieving existing information on their quality. With these goals in mind, here we describe in detail the various tools we developed and the status of the collection, which is currently composed of 11,491 lines and covering 71\% of Drosophila genes. Data on the characterization of the lines either by RT-qPCR or phenotype is available on a dedicated website, the RNAi Stock Validation and Phenotypes Project (RSVP, http://www.flyrnai.org/RSVP.html), and stocks are available from three stock centers, the Bloomington Drosophila Stock Center (United States), National Institute of Genetics (Japan), and TsingHua Fly Center (China).

}, issn = {1943-2631}, doi = {10.1534/genetics.115.180208}, author = {Perkins, Lizabeth A and Holderbaum, Laura and Tao, Rong and Hu, Yanhui and Sopko, Richelle and McCall, Kim and Yang-Zhou, Donghui and Flockhart, Ian and Binari, Richard and Shim, Hye-Seok and Miller, Audrey and Housden, Amy and Foos, Marianna and Randkelv, Sakara and Kelley, Colleen and Namgyal, Pema and Villalta, Christians and Liu, Lu-Ping and Jiang, Xia and Huan-Huan, Qiao and Wang, Xia and Fujiyama, Asao and Toyoda, Atsushi and Ayers, Kathleen and Blum, Allison and Czech, Benjamin and Neumuller, Ralph and Yan, Dong and Cavallaro, Amanda and Hibbard, Karen and Hall, Don and Cooley, Lynn and Hannon, Gregory J and Lehmann, Ruth and Parks, Annette and Mohr, Stephanie E and Ueda, Ryu and Kondo, Shu and Ni, Jian-Quan and Perrimon, Norbert} }