@article {825821,
	title = {Reagent and Data Resources for Investigation of RNA Binding Protein Functions in Drosophila melanogaster Cultured Cells.},
	journal = {G3 (Bethesda)},
	volume = {5},
	year = {2015},
	month = {2015 Sep},
	pages = {1919-24},
	abstract = {<p>RNA binding proteins (RBPs) are involved in many cellular functions. To facilitate functional characterization of RBPs, we generated an RNA interference (RNAi) library for Drosophila cell-based screens comprising reagents targeting known or putative RBPs. To test the quality of the library and provide a baseline analysis of the effects of the RNAi reagents on viability, we screened the library using a total ATP assay and high-throughput imaging in Drosophila S2R+ cultured cells. The results are consistent with production of a high-quality library that will be useful for functional genomics studies using other assays. Altogether, we provide resources in the form of an initial curated list of Drosophila RBPs; an RNAi screening library we expect to be used with additional assays that address more specific biological questions; and total ATP and image data useful for comparison of those additional assay results with fundamental information such as effects of a given reagent in the library on cell viability. Importantly, we make the baseline data, including more than 200,000 images, easily accessible online.</p>},
	issn = {2160-1836},
	doi = {10.1534/g3.115.019364},
	author = {Mohr, Stephanie E and Hu, Yanhui and Rudd, Kirstin and Buckner, Michael and Gilly, Quentin and Foster, Blake and Sierzputowska, Katarzyna and Comjean, Aram and Ye, Bing and Perrimon, Norbert}
}
