@article {825511,
	title = {Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter.},
	journal = {Science},
	volume = {326},
	year = {2009},
	month = {2009 Oct 2},
	pages = {144-7},
	abstract = {<p>Mitochondria are integral components of cellular calcium (Ca2+) signaling. Calcium stimulates mitochondrial adenosine 5{\textquoteright}-triphosphate production, but can also initiate apoptosis. In turn, cytoplasmic Ca2+ concentrations are regulated by mitochondria. Although several transporter and ion-channel mechanisms have been measured in mitochondria, the molecules that govern Ca2+ movement across the inner mitochondrial membrane are unknown. We searched for genes that regulate mitochondrial Ca2+ and H+ concentrations using a genome-wide Drosophila RNA interference (RNAi) screen. The mammalian homolog of one Drosophila gene identified in the screen, Letm1, was found to specifically mediate coupled Ca2+/H+ exchange. RNAi knockdown, overexpression, and liposome reconstitution of the purified Letm1 protein demonstrate that Letm1 is a mitochondrial Ca2+/H+ antiporter.</p>},
	keywords = {Animals, Antiporters, calcium, Calcium-Binding Proteins, Cation Transport Proteins, Cell Line, drosophila melanogaster, Drosophila Proteins, Genome, Human, Genome, Insect, HeLa Cells, Humans, Hydrogen, Hydrogen-Ion Concentration, Ion Transport, Membrane Potential, Mitochondrial, Membrane Proteins, mitochondria, Mitochondrial Membranes, Mitochondrial Proteins, Proteolipids, RNA Interference},
	issn = {1095-9203},
	doi = {10.1126/science.1175145},
	author = {Jiang, Dawei and Zhao, Linlin and Clapham, David E}
}
