@article {825476,
	title = {Genome-wide analysis reveals a cell cycle-dependent mechanism controlling centromere propagation.},
	journal = {J Cell Biol},
	volume = {183},
	year = {2008},
	month = {2008 Dec 1},
	pages = {805-18},
	abstract = {<p>Centromeres are the structural and functional foundation for kinetochore formation, spindle attachment, and chromosome segregation. In this study, we isolated factors required for centromere propagation using genome-wide RNA interference screening for defects in centromere protein A (CENP-A; centromere identifier [CID]) localization in Drosophila melanogaster. We identified the proteins CAL1 and CENP-C as essential factors for CID assembly at the centromere. CID, CAL1, and CENP-C coimmunoprecipitate and are mutually dependent for centromere localization and function. We also identified the mitotic cyclin A (CYCA) and the anaphase-promoting complex (APC) inhibitor RCA1/Emi1 as regulators of centromere propagation. We show that CYCA is centromere localized and that CYCA and RCA1/Emi1 couple centromere assembly to the cell cycle through regulation of the fizzy-related/CDH1 subunit of the APC. Our findings identify essential components of the epigenetic machinery that ensures proper specification and propagation of the centromere and suggest a mechanism for coordinating centromere inheritance with cell division.</p>},
	keywords = {Anaphase-Promoting Complex-Cyclosome, Animals, Annexin A2, Cell Cycle, Cell Cycle Proteins, Cell Line, Centromere, Chromosomal Proteins, Non-Histone, Chromosome Segregation, Cyclin A, DNA-Binding Proteins, drosophila melanogaster, Drosophila Proteins, Epigenesis, Genetic, Genome-Wide Association Study, Histones, Mitosis, Mutation, RNA Interference, S100 Proteins, Ubiquitin-Protein Ligase Complexes},
	issn = {1540-8140},
	doi = {10.1083/jcb.200806038},
	author = {Erhardt, Sylvia and Mellone, Barbara G and Betts, Craig M and Zhang, Weiguo and Karpen, Gary H and Straight, Aaron F}
}
