@article {825296, title = {Drosophila RNAi screen reveals CD36 family member required for mycobacterial infection.}, journal = {Science}, volume = {309}, year = {2005}, month = {2005 Aug 19}, pages = {1251-3}, abstract = {

Certain pathogens, such as Mycobacterium tuberculosis, survive within the hostile intracellular environment of a macrophage. To identify host factors required for mycobacterial entry and survival within macrophages, we performed a genomewide RNA interference screen in Drosophila macrophage-like cells, using Mycobacterium fortuitum. We identified factors required for general phagocytosis, as well as those needed specifically for mycobacterial infection. One specific factor, Peste (Pes), is a CD36 family member required for uptake of mycobacteria, but not Escherichia coli or Staphylococcus aureus. Moreover, mammalian class B scavenger receptors (SRs) conferred uptake of bacteria into nonphagocytic cells, with SR-BI and SR-BII uniquely mediating uptake of M. fortuitum, which suggests a conserved role for class B SRs in pattern recognition and innate immunity.

}, keywords = {Animals, Antigens, CD36, Cell Line, Cytoskeleton, drosophila melanogaster, Escherichia coli, Humans, Immunity, Innate, Lysosome-Associated Membrane Glycoproteins, Macrophages, Membrane Proteins, Mice, Mycobacterium fortuitum, Phagocytosis, Receptors, Immunologic, Receptors, Scavenger, RNA Interference, RNA, Double-Stranded, Scavenger Receptors, Class B, Sialoglycoproteins, Staphylococcus aureus, Transfection, Transport Vesicles}, issn = {1095-9203}, doi = {10.1126/science.1116006}, author = {Philips, Jennifer A and Rubin, Eric J and Perrimon, Norbert} }