@article {825281, title = {Genome-wide RNAi screen reveals a specific sensitivity of IRES-containing RNA viruses to host translation inhibition.}, journal = {Genes Dev}, volume = {19}, year = {2005}, month = {2005 Feb 15}, pages = {445-52}, abstract = {

The widespread class of RNA viruses that utilize internal ribosome entry sites (IRESs) for translation include poliovirus and Hepatitis C virus. To identify host factors required for IRES-dependent translation and viral replication, we performed a genome-wide RNAi screen in Drosophila cells infected with Drosophila C virus (DCV). We identified 66 ribosomal proteins that, when depleted, specifically inhibit DCV growth, but not a non-IRES-containing RNA virus. Moreover, treatment of flies with a translation inhibitor is protective in vivo. Finally, this increased sensitivity to ribosome levels also holds true for poliovirus infection of human cells, demonstrating the generality of these findings.

}, keywords = {Animals, Base Sequence, DNA Primers, Drosophila, Genome, Viral, HeLa Cells, Humans, Protein Biosynthesis, RNA Interference, RNA Viruses, Virus Replication}, issn = {0890-9369}, doi = {10.1101/gad.1267905}, author = {Cherry, Sara and Doukas, Tammy and Armknecht, Susan and Whelan, Sean and Wang, Hui and Sarnow, Peter and Perrimon, Norbert} }