@article {825266,
	title = {Genome-wide RNAi analysis of growth and viability in Drosophila cells.},
	journal = {Science},
	volume = {303},
	year = {2004},
	month = {2004 Feb 6},
	pages = {832-5},
	abstract = {<p>A crucial aim upon completion of whole genome sequences is the functional analysis of all predicted genes. We have applied a high-throughput RNA-interference (RNAi) screen of 19,470 double-stranded (ds) RNAs in cultured cells to characterize the function of nearly all (91\%) predicted Drosophila genes in cell growth and viability. We found 438 dsRNAs that identified essential genes, among which 80\% lacked mutant alleles. A quantitative assay of cell number was applied to identify genes of known and uncharacterized functions. In particular, we demonstrate a role for the homolog of a mammalian acute myeloid leukemia gene (AML1) in cell survival. Such a systematic screen for cell phenotypes, such as cell viability, can thus be effective in characterizing functionally related genes on a genome-wide scale.</p>},
	keywords = {Animals, Apoptosis, Cell Cycle, Cell Survival, Cells, Cultured, Computational Biology, Core Binding Factor Alpha 2 Subunit, DNA-Binding Proteins, drosophila melanogaster, Drosophila Proteins, Genes, Essential, Genes, Insect, genome, Humans, Inhibitor of Apoptosis Proteins, Phenotype, Proteome, Proto-Oncogene Proteins, Reproducibility of Results, RNA Interference, RNA, Double-Stranded, Sequence Homology, Transcription Factors},
	issn = {1095-9203},
	doi = {10.1126/science.1091266},
	author = {Boutros, Michael and Kiger, Amy A and Armknecht, Susan and Kerr, Kim and Hild, Marc and Koch, Britta and Haas, Stefan A and Paro, Renato and Perrimon, Norbert and Heidelberg Fly Array Consortium}
}
