@article {Viswanatha2021.03.29.437496,
	title = {Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos [NOTE: A modified final version was published in Nat Comm and is now available.]},
	journal = {bioRxiv},
	year = {2021},
	publisher = {Cold Spring Harbor Laboratory},
	abstract = {Mosquito-borne diseases present a worldwide public health burden. Genome-scale screening tools that could inform our understanding of mosquitos and their control are lacking. Here, we adapt a recombination-mediated cassette exchange system for delivery of CRISPR sgRNA libraries into cell lines from several mosquito species and perform pooled CRISPR screens in an Anopheles cell line. To implement this method, we engineered modified mosquito cell lines, validated promoters and developed bioinformatics tools for multiple mosquito species.Competing Interest StatementThe authors have declared no competing interest.},
	doi = {10.1101/2021.03.29.437496},
	url = {https://www.biorxiv.org/content/early/2021/03/30/2021.03.29.437496},
	author = {Viswanatha, Raghuvir and Mameli, Enzo and Rodiger, Jonathan and Merckaert, Pierre and Feitosa-Suntheimer, Fabiana and Colpitts, Tonya M. and Mohr, Stephanie E. and Hu, Yanhui and Perrimon, Norbert}
}
