@article {1291760,
	title = {Large-Scale Transgenic Resource Collections for Loss- and Gain-of-Function Studies},
	journal = {Genetics},
	year = {2020},
	month = {2020 Feb 18},
	abstract = {The Transgenic RNAi Project (TRiP), a functional genomics platform at Harvard Medical School, was initiated in 2008 to generate and distribute a genome-scale collection of RNAi fly stocks. To date, the TRiP has generated \&gt;15,000 RNAi fly stocks. As this covers most genes, we have largely transitioned to development of new resources based on CRISPR technology. Here, we present an update on our libraries of publicly available RNAi and CRISPR fly stocks, and focus on the TRiP-CRISPR overexpression (TRiP-OE) and TRiP-CRISPR knockout (TRiP-KO) collections. TRiP-OE stocks express sgRNAs targeting upstream of a gene transcription start site. Gene activation is triggered by co-expression of catalytically dead Cas9 (dCas9) fused to an activator domain, either VP64-p65-Rta (VPR) or Synergistic Activation Mediator (SAM). TRiP-KO stocks express one or two sgRNAs targeting the coding sequence of a gene or genes. Cutting is triggered by co-expression of Cas9, allowing for generation of indels in both germline and somatic tissue. To date, we have generated more than 5,000 CRISPR-OE or -KO stocks for the community. These resources provide versatile, transformative tools for gene activation, gene repression, and genome engineering.},
	issn = {1943-2631},
	doi = {10.1534/genetics.119.302964},
	author = {Zirin, Jonathan and Hu, Yanhui and Liu, Luping and Yang-Zhou, Donghui and Colbeth, Ryan and Yan, Dong and Ewen-Campen, Ben and Tao, Rong and Vogt, Eric and VanNest, Sara and Cavers, Cooper and Villalta, Christians and Comjean, Aram and Sun, Jin and Wang, Xia and Jia, Yu and Zhu, Ruibao and Peng, Ping and Yu, Jinchao and Shen, Da and Qiu, Yuhao and Ayisi, Limmond and Ragoowansi, Henna and Fenton, Ethan and Efrem, Senait and Parks, Annette and Saito, Kuniaki and Kondo, Shu and Perkins, Liz and Mohr, Stephanie E and Ni, Jianquan and Perrimon, Norbert}
}
