@article {1137727,
	title = {Ex vivo genome-wide RNAi screening of the Drosophila Toll signaling pathway elicited by a larva-derived tissue extract},
	journal = {Biochem Biophys Res Commun},
	volume = {467},
	year = {2015},
	month = {2015 Nov 13},
	pages = {400-6},
	abstract = {Damage-associated molecular patterns (DAMPs), so-called "danger signals," play important roles in host defense and pathophysiology in mammals and insects. In Drosophila, the Toll pathway confers damage responses during bacterial infection and improper cell-fate control. However, the intrinsic ligands and signaling mechanisms that potentiate innate immune responses remain unknown. Here, we demonstrate that a Drosophila larva-derived tissue extract strongly elicits Toll pathway activation via the Toll receptor. Using this extract, we performed ex\&nbsp;vivo genome-wide RNAi screening in Drosophila cultured cells, and identified several signaling factors that are required for host defense and antimicrobial-peptide expression in Drosophila adults. These results suggest that our larva-derived tissue extract contains active ingredients that mediate Toll pathway activation, and the screening data will shed light on the mechanisms of damage-related Toll pathway signaling in Drosophila.},
	keywords = {Adaptor Proteins, Signal Transducing, Animals, Antigens, Differentiation, Antimicrobial Cationic Peptides, Cells, Cultured, drosophila melanogaster, Drosophila Proteins, Gene Expression Regulation, Genes, Reporter, High-Throughput Screening Assays, Immunity, Innate, Larva, Luciferases, Protein-Serine-Threonine Kinases, Receptors, Immunologic, RNA Interference, Signal Transduction, Staphylococcus saprophyticus, Tissue Extracts, toll-like receptors},
	issn = {1090-2104},
	doi = {10.1016/j.bbrc.2015.09.138},
	author = {Kanoh, Hirotaka and Kuraishi, Takayuki and Tong, Li-Li and Watanabe, Ryo and Nagata, Shinji and Kurata, Shoichiro}
}
