#  Protocols 

 



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## Protocols for *in vivo* studies include

- [RNAi](/trip-vivo-rnai-approach)
- [CRISPR approaches](/vivo-crispr-0)
- [overexpression](/vivo-fly-approaches)

## Protocols for cell-based approaches include

- [single-cell isolation](/single-cell-isolation)
- [making stable cell lines](/stable-fly-cell-lines)
- [isolation of primary cells from embryos](/fly-primary-cells)
- [fix and stain for cell imaging](/fly-cell-staining-dapi-and-phalloidin)
- treatment of cells with RNAi ([6-well format](/fly-cell-RNAi-6-well-format); [384-well format](/fly-cell-rnai-384-well-format))

## Looking in general for *Drosophila* protocols?

Check out our beta test of a [*Drosophila* protocols portal](/beta-test-site-drosophila-protocols-portal).