#  Variable Dose Analysis: a new DRSC-supported cell screen approach that leverages existing reagents to perform robust screens 

 





December 01, 2017

 

 

 We are excited to report the publication of a paper from Benjamin Housden and colleagues describing development and use of the Variable Dose Analysis (VDA) approach. Ben developed a way to use existing TRiP shRNA plasmids originally developed for fly stock production in a new, effective approach to high-throughput cell screening.

 The VDA approach is particularly useful for combinatorial approaches that are acutely sensitive to assay robustness. The screen Ben and colleagues report focused on synthetic effects in *Drosophila* tumor model cells. The approach should be generally applicable to similar high-throughput screens in other cell types.

 The DRSC provides support for VDA screening in Drosophila cells. Please contact the Director for more information or to begin discussing a screen assay.

 Housden BE, Li Z, Kelley C, Wang Y, Hu Y, Valvezan AJ, Manning BD, Perrimon N. **Improved detection of synthetic lethal interactions in Drosophila cells using variable dose analysis (VDA).** Proc Natl Acad Sci U S A. 2017 Nov 28. pii: 201713362. doi: 10.1073/pnas.1713362114. PMID: [29183982](https://www.ncbi.nlm.nih.gov/pubmed/29183982).



 

 

 

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 Attachments- [  picture\_as\_pdf  pnas-2017-housden-1713362114.pdf ](/sites/g/files/omnuum5366/files/fly/files/pnas-2017-housden-1713362114.pdf)
 
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 See also:- [ Drosophila (fly) ](/organism/drosophila-fly)
- [ Human ](/organism/human)
- [ Mouse ](/organism/mouse)
- [ Cell-based assays ](/technology/cell-based-assays)
- [ Cell-based RNAi ](/technology/fly-cell-based-rnai)
- [ High-throughput data analysis ](/topics/high-throughput-data-analysis)
- [ Screening technologies ](/topics/screening-technologies)
- [ Synthetic lethality ](/topics/synthetic-lethality)
 
 

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