#  Pooled-format CRISPR screens in Drosophila cells 

 





March 22, 2018

 

 

 The DRSC/TRiP-FGR is pleased to support collaborations on pooled CRISPR screens using the method recently, [reported in eLife by Viswanatha et al](https://elifesciences.org/articles/36333). (PDF download file below).

 From the abstract: "... Here, we developed a site-specific integration strategy for library delivery and performed a genome-wide CRISPR knockout screen in Drosophila S2R+ cells. Under basal growth conditions, 1235 genes were essential for cell fitness at a false-discovery rate of 5% ... We additionally performed context-specific fitness screens for resistance to or synergy with trametinib, a Ras/ERK/ETS inhibitor, or rapamycin, an mTOR inhibitor, and identified key regulators of each pathway. The results present a novel, scalable, and versatile platform for functional genomic screens in low-redundancy animal cells."

 Interested to design a CRISPR pooled drop-out or selection screen to interrogate a cellular function you are interested in? Please contact the Director S. Mohr. We are actively seeking collaborations on this exciting new approach.

 *See also this November 2018 report in which CRISPR pooled-format screening identifies a transporter for the steroid hormone ecdysone:*

Okamoto N, Viswanatha R, Bittar R, Li Z, Haga-Yamanaka S, Perrimon N, Yamanaka N. **A Membrane Transporter Is Required for Steroid Hormone Uptake in Drosophila.** Dev Cell. 2018 Nov 5;47(3):294-305.e7. doi: 10.1016/j.devcel.2018.09.012. Epub2018 Oct 4. PubMed PMID: [30293839](https://ncbi.nlm.nih.gov/pubmed/30293839)

 

 

 

---

 Attachments- [  picture\_as\_pdf  274464.full\_.pdf ](/sites/g/files/omnuum5366/files/fly/files/274464.full_.pdf)
 
---

 



 

 See also:- [ Drosophila (fly) ](/organism/drosophila-fly)
- [ Cell-based assays ](/technology/cell-based-assays)
- [ CRISPR gRNA design ](/technology/crispr-grna-design)
- [ Cell-based CRISPR ](/technology/fly-cell-based-crispr)
- [ Data mining ](/topics/data-mining)
- [ Essential genes ](/topics/essential-genes)
- [ Genome-wide screen ](/topics/genome-wide-screen)
 
 

 Share on:- [     Facebook ](#)
- [     Twitter ](#)
- [     Linkedin ](#)