#  FlyPrimerBank 

 



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FlyPrimerBank provides a resource of pre-computed primers appropriate for qPCR. The primer designs were based on the original PrimerBank algorithms as well as our own internal algorithms. Key information such as spanning of intron-exon boundaries and availability of validation data are included in search results.

## URL

<http://www.flyrnai.org/flyprimerbank>

## Version

1.0 (includes auto ID update)

## Tutorial

To request a tutorial video, please [make the suggestion on our bug report form](https://hms.az1.qualtrics.com/jfe/form/SV_cApK7Mvnw54HeyV). Please also visit the FlyPrimerBank documentation page, or read our paper (see below), for help with the tool.

## Documentation

Please visit the [FlyPrimerBank documentation page](/flyprimerbank-documentation).

## Additional Resources

Those interested in qPCR primer designs for mouse and human genes should visit [PrimerBank](https://pga.mgh.harvard.edu/primerbank/).

Have you used FlyPrimerBank to do qPCR and seen success (or not) with primers? Please consider [submitting your feedback on primer designs](/flyprimerbank-addfeedback). Validation data for in vivo RNAi fly stocks, including qPCR data, can be viewed and/or submitted at [RSVP](/rsvp).

## [Report a Bug](https://hms.az1.qualtrics.com/jfe/form/SV_cApK7Mvnw54HeyV)