#  RNAi rescue reagents 

 



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RNAi rescue provides a test for on-target specificity of an RNAi reagent. With RNAi rescue, activity of the presumed target gene is provided via a transgene that provides wildtype function but is not recognized by the RNAi reagent (mutant or different in nucleotide sequence in the target region).

For *Drosophila*, RNAi rescue can be achieved using a cross-species approach. Related *Drosophila* species sit in a 'sweet spot' for some *D. melanogaster* genes, in which function is conserved but the nucleotide sequence is divergent enough that the non-D. mel species transcripts evade RNAi.

DRSC-FGR has a *D. persimilis* clone collection available for an RNAi rescue approach. Use our [online portal](http://www.flyrnai.org/cgi-bin/RNAi_find_rescue_compl.pl) to identify appropriate clones in the D. persimilis collection, as well as in *D. virilis* and *D. psuedoobscura* collections available from other resource centers.