# in vivo fly RNAi (TRiP) ## expand\_more [**Quick link to GeneLookup (search DRSC/TRiP reagents)**](http://www.flyrnai.org/cgi-bin/DRSC_gene_lookup.pl) [**Quick link to UP-TORR (batch search fly RNAi reagents)**](http://www.flyrnai.org/up-torr/) [**Quick link to RSVP (search in vivo fly RNAi data)**](http://www.flyrnai.org/cgi-bin/RSVP_search.pl) [**Quick link to TRiP fly stock batch query**](http://www.flyrnai.org/cgi-bin/TRiP_batch_query.pl) Since 2008, the Transgenic RNAi Project (TRiP) has generated transgenic RNAi fly stocks based on our optimized approach that are made immediately and openly available to the community through the [Bloomington Drosophila Stock Center](https://bdsc.indiana.edu/index.html) (BDSC). - learn about [TRiP RNAi fly stocks](/trip-in-vivo-fly-rnai) (reagents) - learn about [the TRiP approach](/trip-vivo-rnai-approach) (protocols) - search for TRiP fly stocks using [UP-TORR](/up-torr) - view and contribute fly stock validation and phenotype data at [RSVP](/rsvp) See links below to relevant online tools, reagents, protocols, publications, and more. ![wt_oocyte_01.jpg](/sites/g/files/omnuum5366/files/fly/files/wt_oocyte_01.jpg) ## Online tools [### UP-TORR ](/up-torr) [### RSVP ](/rsvp) [### FlyPrimerBank ](/flyprimerbank) [### TRiP fly stock batch query ](/trip-fly-stock-batch-query) ## Reagents [### in vivo RNAi fly stocks and vectors ](/trip-in-vivo-fly-rnai) [### RNAi rescue reagents ](/fly-rnai-rescue) ## Protocols [### in vivo RNAi ](/trip-vivo-rnai-approach) [### UAS overexpression ](/vivo-fly-approaches) ## News [### Important new considerations for TRiP stock users ](/news/important-new-considerations-trip-stock-users) August 29, 2022 [### DRSC/TRiP and DRSC-BTRR Office Hours ](/OfficeHours2021) September 13, 2021 [### DRSC-Biomedical Technology Research Resource ](/news/drsc-biomedical-technology-research-resource) October 21, 2019 [### Micropublication relevant to TRiP fly stocks ](/news/micropublication-relevant-trip-fly-stocks) April 09, 2019 [### New stocks added to the TRiP in vivo RNAi library ](/news/new-stocks-added-trip-vivo-rnai-library) January 06, 2017 [### Is it a hit? On mining our data sets. ](/news/it-hit-mining-our-data-sets) July 22, 2016 ## Contact Us Please [contact us](/contact) for any questions. ## Publications Download 6 citations download- [BibTeX](/bibcite/export?pager_style=no_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114101&&&format=bibtex) - [EndNote X3 XML](/bibcite/export?pager_style=no_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114101&&&format=endnote8) - [EndNote 7 XML](/bibcite/export?pager_style=no_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114101&&&format=endnote7) - [Endnote tagged](/bibcite/export?pager_style=no_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114101&&&format=tagged) - [Marc](/bibcite/export?pager_style=no_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114101&&&format=marc) - [PubMedId](/bibcite/export?pager_style=no_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114101&&&format=pubmed_id) - [RIS](/bibcite/export?pager_style=no_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114101&&&format=ris) ### 2021 Xiangzhao Yue, Yongkang Liang, Zhishuang Wei, Jun Lv, Yongjin Cai, Xiaobin Fan, Wenqing Zhang, and Jie Chen. 2021. “[Genome-Wide in Vitro and in Vivo RNAi Screens Reveal Fer3 to Be an Important Regulator of Kkv Transcription in Drosophila](/publications/genome-wide-vitro-and-vivo-rnai-screens-reveal-fer3-be-important-regulator-kkv)”. Insect Sci. doi:10.1111/1744-7917.12954 Xiangzhao Yue, Yongkang Liang, Zhishuang Wei, Jun Lv, Yongjin Cai, Xiaobin Fan, Wenqing Zhang, and Jie Chen. 2021. “[Genome-Wide in Vitro and in Vivo RNAi Screens Reveal Fer3 to Be an Important Regulator of Kkv Transcription in Drosophila](/publications/genome-wide-vitro-and-vivo-rnai-screens-reveal-fer3-be-important-regulator-kkv)”. Insect Sci. doi:10.1111/1744-7917.12954 - add\_circle do\_not\_disturb\_on Abstract Krotzkopf verkehrt (kkv) is a key enzyme that catalyzes the synthesis of chitin, an important component of the Drosophila epidermis, trachea, and other tissues. Here, we report the use of comprehensive RNA interference (RNAi) analyses to search for kkv... ### 2020 Yanhui Hu, Aram Comjean, Jonathan Rodiger, Yifang Liu, Yue Gao, Verena Chung, Jonathan Zirin, Norbert Perrimon, and Stephanie Mohr. 2020. “[FlyRNAi.Org-the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2021 Update](/publications/flyrnaiorg-database-drosophila-rnai-screening-center-and-transgenic-rnai-project)”. Nucleic Acids Res. doi:10.1093/nar/gkaa936 Yanhui Hu, Aram Comjean, Jonathan Rodiger, Yifang Liu, Yue Gao, Verena Chung, Jonathan Zirin, Norbert Perrimon, and Stephanie Mohr. 2020. “[FlyRNAi.Org-the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2021 Update](/publications/flyrnaiorg-database-drosophila-rnai-screening-center-and-transgenic-rnai-project)”. Nucleic Acids Res. doi:10.1093/nar/gkaa936 - add\_circle do\_not\_disturb\_on Abstract - [ picture\_as\_pdfgkaa936.pdf](/sites/g/files/omnuum5366/files/fly/files/gkaa936.pdf) The FlyRNAi database at the Drosophila RNAi Screening Center and Transgenic RNAi Project (DRSC/TRiP) provides a suite of online resources that facilitate functional genomics studies with a special emphasis on Drosophila melanogaster. Currently, the... - [ picture\_as\_pdfgkaa936.pdf](/sites/g/files/omnuum5366/files/fly/files/gkaa936.pdf) Jonathan Zirin, Yanhui Hu, Luping Liu, Donghui Yang-Zhou, Ryan Colbeth, Dong Yan, Ben Ewen-Campen, Rong Tao, Eric Vogt, Sara VanNest, Cooper Cavers, Christians Villalta, Aram Comjean, Jin Sun, Xia Wang, Yu Jia, Ruibao Zhu, Ping Peng, Jinchao Yu, Da Shen, Yuhao Qiu, Limmond Ayisi, Henna Ragoowansi, Ethan Fenton, Senait Efrem, Annette Parks, Kuniaki Saito, Shu Kondo, Liz Perkins, Stephanie Mohr, Jianquan Ni, and Norbert Perrimon. 2020. “[Large-Scale Transgenic Resource Collections for Loss- and Gain-of-Function Studies](/publications/large-scale-transgenic-resource-collections-loss-and-gain-function-studies)”. Genetics. doi:10.1534/genetics.119.302964 Jonathan Zirin, Yanhui Hu, Luping Liu, Donghui Yang-Zhou, Ryan Colbeth, Dong Yan, Ben Ewen-Campen, Rong Tao, Eric Vogt, Sara VanNest, Cooper Cavers, Christians Villalta, Aram Comjean, Jin Sun, Xia Wang, Yu Jia, Ruibao Zhu, Ping Peng, Jinchao Yu, Da Shen, Yuhao Qiu, Limmond Ayisi, Henna Ragoowansi, Ethan Fenton, Senait Efrem, Annette Parks, Kuniaki Saito, Shu Kondo, Liz Perkins, Stephanie Mohr, Jianquan Ni, and Norbert Perrimon. 2020. “[Large-Scale Transgenic Resource Collections for Loss- and Gain-of-Function Studies](/publications/large-scale-transgenic-resource-collections-loss-and-gain-function-studies)”. Genetics. doi:10.1534/genetics.119.302964 - add\_circle do\_not\_disturb\_on Abstract The Transgenic RNAi Project (TRiP), a functional genomics platform at Harvard Medical School, was initiated in 2008 to generate and distribute a genome-scale collection of RNAi fly stocks. To date, the TRiP has generated >15,000 RNAi fly stocks. As this... ### 2019 Michael Rotelli, Anna Bolling, Andrew Killion, Abraham Weinberg, Michael Dixon, and Brian Calvi. 2019. “[An RNAi Screen for Genes Required for Growth of Wing Tissue](/publications/rnai-screen-genes-required-growth-wing-tissue)”. G3 (Bethesda), 9, 10, Pp. 3087-3100. doi:10.1534/g3.119.400581 Michael Rotelli, Anna Bolling, Andrew Killion, Abraham Weinberg, Michael Dixon, and Brian Calvi. 2019. “[An RNAi Screen for Genes Required for Growth of Wing Tissue](/publications/rnai-screen-genes-required-growth-wing-tissue)”. G3 (Bethesda), 9, 10, Pp. 3087-3100. doi:10.1534/g3.119.400581 - add\_circle do\_not\_disturb\_on Abstract - [ picture\_as\_pdf3087.full\_.pdf](/sites/g/files/omnuum5366/files/fly/files/3087.full_.pdf) Cell division and tissue growth must be coordinated with development. Defects in these processes are the basis for a number of diseases, including developmental malformations and cancer. We have conducted an unbiased RNAi screen for genes that are... - [ picture\_as\_pdf3087.full\_.pdf](/sites/g/files/omnuum5366/files/fly/files/3087.full_.pdf) Stephanie E. Mohr and Norbert Perrimon. 2019. “[Drosophila Melanogaster: A Simple System for Understanding Complexity.](/publications/drosophila-melanogaster-simple-system-understanding-complexity)”. Dis Model Mech, 12, 10 Stephanie E. Mohr and Norbert Perrimon. 2019. “[Drosophila Melanogaster: A Simple System for Understanding Complexity.](/publications/drosophila-melanogaster-simple-system-understanding-complexity)”. Dis Model Mech, 12, 10 - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](https://dmm.biologists.org/content/12/10/dmm041871.long) - [ picture\_as\_pdf2019\_DMM\_Mohr.pdf](/sites/g/files/omnuum5366/files/fly/files/2019_DMM_Mohr.pdf) Understanding human gene function is fundamental to understanding and treating diseases. Research using the model organism *Drosophila melanogaster* benefits from a wealth of molecular genetic resources and information useful for efficient *in* *vivo* experimen... - [ descriptionPublisher's Version](https://dmm.biologists.org/content/12/10/dmm041871.long) - [ picture\_as\_pdf2019\_DMM\_Mohr.pdf](/sites/g/files/omnuum5366/files/fly/files/2019_DMM_Mohr.pdf) ### 2016 Benjamin Housden, Matthias Muhar, Matthew Gemberling, Charles Gersbach, Didier YR Stainier, Geraldine Seydoux, Stephanie Mohr, Johannes Zuber, and Norbert Perrimon. 2016. “[Loss-of-Function Genetic Tools for Animal Models: Cross-Species and Cross-Platform Differences](/publications/loss-function-genetic-tools-animal-models-cross-species-and-cross-platform)”. Nat Rev Genet Benjamin Housden, Matthias Muhar, Matthew Gemberling, Charles Gersbach, Didier YR Stainier, Geraldine Seydoux, Stephanie Mohr, Johannes Zuber, and Norbert Perrimon. 2016. “[Loss-of-Function Genetic Tools for Animal Models: Cross-Species and Cross-Platform Differences](/publications/loss-function-genetic-tools-animal-models-cross-species-and-cross-platform)”. Nat Rev Genet - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](https://www.ncbi.nlm.nih.gov/pubmed/27795562) - [ picture\_as\_pdf2016\_Nat Rev Gene\_Housden...](/sites/g/files/omnuum5366/files/fly/files/2016_NatRevGene_Housden.pdf) Our understanding of the genetic mechanisms that underlie biological processes has relied extensively on loss-of-function (LOF) analyses. LOF methods target DNA, RNA or protein to reduce or to ablate gene function. By analysing the phenotypes that are... - [ descriptionPublisher's Version](https://www.ncbi.nlm.nih.gov/pubmed/27795562) - [ picture\_as\_pdf2016\_Nat Rev Gene\_Housden...](/sites/g/files/omnuum5366/files/fly/files/2016_NatRevGene_Housden.pdf) [ More arrow\_circle\_right ](/publications/technology/in-vivo-fly-rnai)