# Cell-based RNAi (DRSC) **[Quick link to Gene Lookup (search screen data)](http://www.flyrnai.org/cgi-bin/DRSC_gene_lookup.pl) [Quick link to UP-TORR (view cell & in vivo reagents)](http://www.flyrnai.org/up-torr/)** *Drosophila* cell-based RNAi screens have been supported by our facility since 2003. We provide reagents targeting individual genes, focused libraries, genome-scale libraries, and other resources for [on-site screening at our facility](/on-site-screens) or off-site screening at your institution. - view our [genome-wide and focused libraries](/fly-cell-rnai-libraries) (reagents) - learn about [making RNAi reagents](/drsc-cell-rnai) or [fly cell culture](/fly-cell-culture) (protocols) - mine data from past screens at [ScreenSummary](/screensummary) See links below to relevant online tools, reagents, protocols, publications, and more. ![plate](/sites/g/files/omnuum5366/files/fly/files/assay-plate.jpg) ## Online tools [### UP-TORR ](/up-torr) Description UP-TORR provides accurate and up-to-date annotation of cell-based and in vivo RNAi reagents targeting Drosophila genes, including reagents from all major public collections: DRSC, DKFZ, NIG-Japan, VDRC and TRiP. UP-TORR can also provide up-to... description [### SnapDragon ](/snapdragon) Description SnapDragon is a web-based tool for the design of double-stranded RNAs (dsRNAs) for RNAi knockdown in Drosophila. URL [http://www.flyrnai.org/cgi-bin/RNAi\_find\_primers.pl](http://www.flyrnai.org/cgi-bin/RNAi_find_primers.pl) Version na Tutorial To request a tutorial video, please make the... description [### FlyPrimerBank ](/flyprimerbank) FlyPrimerBank provides a resource of pre-computed primers appropriate for qPCR. The primer designs were based on the original PrimerBank algorithms as well as our own internal algorithms. Key information such as spanning of intron-exon boundaries and... description ## Reagents [### Cell RNAi libraries ](/fly-cell-rnai-libraries) We have an extensive collection of DNA templates for production of double stranded RNA (dsRNA) via in vitro transcription, as well as many assay-ready libraries for RNAi screening of Drosophila cells using dsRNAs. Cell RNAi reagents and libraries are now... description [### RNAi rescue reagents ](/fly-rnai-rescue) RNAi rescue provides a test for on-target specificity of an RNAi reagent. With RNAi rescue, activity of the presumed target gene is provided via a transgene that provides wildtype function but is not recognized by the RNAi reagent (mutant or different in... description ## Protocols [### Cell RNAi (6-well) ](/fly-cell-RNAi-6-well-format) This protocol is for RNAi screening of Drosophila cells in 6-well plates using the “bathing” method of delivery (no transfection reagent). We also provide a protocol for RNAi screening in 384-well plates using the bathing method or a transfection method... description [### Cell RNAi (384-well) ](/fly-cell-rnai-384-well-format) This protocol is for RNAi screening with Drosophila cells in 384-well micro-well plates, with additional information on transfection-based RNAi in 96-well format. 384-well format is the format used for RNAi reagents in the DRSC 2.0 genome-wide and other... description [### dsRNA synthesis ](/dsrna-synthesis) Protocol for dsRNA Synthesis We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends. It is also possible to produce dsRNA using PCR generated DNA templates containing either... description ## News [### Read the DRSC/TRiP Report to the Fly Board 2022 ](/news/read-drsctrip-report-fly-board-2022) March 31, 2022 Below we share with the community at large the report we provided to the Fly Board in advance of the 2022 Fly Meeting. As always, your feedback on our technologies and services is welcome. We are here to help. DRSC/TRiP Functional Genomics Resources at... ![Decorative cartoon drawn with BioRender depicting DRSC-BTRR technology concepts](/sites/g/files/omnuum5366/files/styles/hwp_16_9__480x270/public/fly/files/drsc-btrr_499-350.jpeg?itok=kyu_pmTs) [### DRSC/TRiP and DRSC-BTRR Office Hours ](/OfficeHours2021) September 13, 2021 New this fall: Online office hours! Do you have questions about modifying Drosophila cell lines with CRISPR or performing large-scale cell screens? Questions about in vivo RNAi with TRiP fly stocks or CRISPR knockout or activation with our sgRNA fly... ![Graphical image of tissue culture, fly pushing, and computer, and the team of people who work with them](/sites/g/files/omnuum5366/files/styles/hwp_16_9__480x270/public/fly/files/team.jpeg?itok=NLIA2Ora) ## Contact Us Please [contact us](/contact) for any questions. ## Publications Download 6 citations download- [BibTeX](/bibcite/export?pager_style=no_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114106&&&format=bibtex) - [EndNote X3 XML](/bibcite/export?pager_style=no_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114106&&&format=endnote8) - [EndNote 7 XML](/bibcite/export?pager_style=no_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114106&&&format=endnote7) - [Endnote tagged](/bibcite/export?pager_style=no_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114106&&&format=tagged) - [Marc](/bibcite/export?pager_style=no_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114106&&&format=marc) - [PubMedId](/bibcite/export?pager_style=no_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114106&&&format=pubmed_id) - [RIS](/bibcite/export?pager_style=no_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114106&&&format=ris) ### 2022 Shue Chen, Leah Rosin, Gianluca Pegoraro, Nellie Moshkovich, Patrick Murphy, Guoyun Yu, and Elissa Lei. 2022. “[NURF301 Contributes to Gypsy Chromatin Insulator-Mediated Nuclear Organization](/publications/nurf301-contributes-gypsy-chromatin-insulator-mediated-nuclear-organization)”. Nucleic Acids Res, 50, 14, Pp. 7906-24. doi:10.1093/nar/gkac600 Shue Chen, Leah Rosin, Gianluca Pegoraro, Nellie Moshkovich, Patrick Murphy, Guoyun Yu, and Elissa Lei. 2022. “[NURF301 Contributes to Gypsy Chromatin Insulator-Mediated Nuclear Organization](/publications/nurf301-contributes-gypsy-chromatin-insulator-mediated-nuclear-organization)”. Nucleic Acids Res, 50, 14, Pp. 7906-24. doi:10.1093/nar/gkac600 - add\_circle do\_not\_disturb\_on Abstract - [ picture\_as\_pdfgkac600.pdf](/sites/g/files/omnuum5366/files/fly/files/gkac600.pdf) Chromatin insulators are DNA-protein complexes that can prevent the spread of repressive chromatin and block communication between enhancers and promoters to regulate gene expression. In Drosophila, the gypsy chromatin insulator complex consists of three... - [ picture\_as\_pdfgkac600.pdf](/sites/g/files/omnuum5366/files/fly/files/gkac600.pdf) Jiunn Song, Arda Mizrak, Chia-Wei Lee, Marcelo Cicconet, Zon Weng Lai, Wei-Chun Tang, Chieh-Han Lu, Stephanie E. Mohr, Robert V. Farese, and Tobias C. Walther. 2022. “[Identification of Two Pathways Mediating Protein Targeting from ER to Lipid Droplets](/publications/identification-two-pathways-mediating-protein-targeting-er-lipid-droplets)”. Nature Cell Biol Jiunn Song, Arda Mizrak, Chia-Wei Lee, Marcelo Cicconet, Zon Weng Lai, Wei-Chun Tang, Chieh-Han Lu, Stephanie E. Mohr, Robert V. Farese, and Tobias C. Walther. 2022. “[Identification of Two Pathways Mediating Protein Targeting from ER to Lipid Droplets](/publications/identification-two-pathways-mediating-protein-targeting-er-lipid-droplets)”. Nature Cell Biol - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](https://doi.org/10.1038/s41556-022-00974-0) - [ picture\_as\_pdfs41556-022-00974-0-1.pdf](/sites/g/files/omnuum5366/files/fly/files/s41556-022-00974-0-1.pdf) Pathways localizing proteins to their sites of action are essential for eukaryotic cell organization and function. Although mechanisms of protein targeting to many organelles have been defined, how proteins, such as metabolic enzymes, target from the... - [ descriptionPublisher's Version](https://doi.org/10.1038/s41556-022-00974-0) - [ picture\_as\_pdfs41556-022-00974-0-1.pdf](/sites/g/files/omnuum5366/files/fly/files/s41556-022-00974-0-1.pdf) ### 2021 Xiangzhao Yue, Yongkang Liang, Zhishuang Wei, Jun Lv, Yongjin Cai, Xiaobin Fan, Wenqing Zhang, and Jie Chen. 2021. “[Genome-Wide in Vitro and in Vivo RNAi Screens Reveal Fer3 to Be an Important Regulator of Kkv Transcription in Drosophila](/publications/genome-wide-vitro-and-vivo-rnai-screens-reveal-fer3-be-important-regulator-kkv)”. Insect Sci. doi:10.1111/1744-7917.12954 Xiangzhao Yue, Yongkang Liang, Zhishuang Wei, Jun Lv, Yongjin Cai, Xiaobin Fan, Wenqing Zhang, and Jie Chen. 2021. “[Genome-Wide in Vitro and in Vivo RNAi Screens Reveal Fer3 to Be an Important Regulator of Kkv Transcription in Drosophila](/publications/genome-wide-vitro-and-vivo-rnai-screens-reveal-fer3-be-important-regulator-kkv)”. Insect Sci. doi:10.1111/1744-7917.12954 - add\_circle do\_not\_disturb\_on Abstract Krotzkopf verkehrt (kkv) is a key enzyme that catalyzes the synthesis of chitin, an important component of the Drosophila epidermis, trachea, and other tissues. Here, we report the use of comprehensive RNA interference (RNAi) analyses to search for kkv... Jiunn Song, Arda Mizrak, Chia-Wei Lee, Marcelo Cicconet, Zon Weng Lai, Chieh-Han Lu, Stephanie E. Mohr, Jr Robert V. Farese, and Tobias C. Walther. 2021. “[Identification of Two Pathways Mediating Protein Targeting from ER to Lipid Droplets \[NOTE: A Modified Final Version Was Published in Nat Cell Biol and Is Now Available\]](/publications/identification-two-pathways-mediating-protein-targeting-erto-lipid-droplets)” Jiunn Song, Arda Mizrak, Chia-Wei Lee, Marcelo Cicconet, Zon Weng Lai, Chieh-Han Lu, Stephanie E. Mohr, Jr Robert V. Farese, and Tobias C. Walther. 2021. “[Identification of Two Pathways Mediating Protein Targeting from ER to Lipid Droplets \[NOTE: A Modified Final Version Was Published in Nat Cell Biol and Is Now Available\]](/publications/identification-two-pathways-mediating-protein-targeting-erto-lipid-droplets)” - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](https://www.biorxiv.org/content/10.1101/2021.09.14.460330v1) - [ picture\_as\_pdf2021.09.14.460330v1.full\_...](/sites/g/files/omnuum5366/files/fly/files/2021.09.14.460330v1.full_.pdf) Pathways localizing proteins to their sites of action within a cell are essential for eukaryotic cell organization and function. Although mechanisms of protein targeting to many organelles have been defined, little is known about how proteins, such as key... - [ descriptionPublisher's Version](https://www.biorxiv.org/content/10.1101/2021.09.14.460330v1) - [ picture\_as\_pdf2021.09.14.460330v1.full\_...](/sites/g/files/omnuum5366/files/fly/files/2021.09.14.460330v1.full_.pdf) ### 2020 Yanhui Hu, Aram Comjean, Jonathan Rodiger, Yifang Liu, Yue Gao, Verena Chung, Jonathan Zirin, Norbert Perrimon, and Stephanie Mohr. 2020. “[FlyRNAi.Org-the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2021 Update](/publications/flyrnaiorg-database-drosophila-rnai-screening-center-and-transgenic-rnai-project)”. Nucleic Acids Res. doi:10.1093/nar/gkaa936 Yanhui Hu, Aram Comjean, Jonathan Rodiger, Yifang Liu, Yue Gao, Verena Chung, Jonathan Zirin, Norbert Perrimon, and Stephanie Mohr. 2020. “[FlyRNAi.Org-the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2021 Update](/publications/flyrnaiorg-database-drosophila-rnai-screening-center-and-transgenic-rnai-project)”. Nucleic Acids Res. doi:10.1093/nar/gkaa936 - add\_circle do\_not\_disturb\_on Abstract - [ picture\_as\_pdfgkaa936.pdf](/sites/g/files/omnuum5366/files/fly/files/gkaa936.pdf) The FlyRNAi database at the Drosophila RNAi Screening Center and Transgenic RNAi Project (DRSC/TRiP) provides a suite of online resources that facilitate functional genomics studies with a special emphasis on Drosophila melanogaster. Currently, the... - [ picture\_as\_pdfgkaa936.pdf](/sites/g/files/omnuum5366/files/fly/files/gkaa936.pdf) ### 2019 Hilary Nicholson, Zeshan Tariq, Benjamin Housden, Rebecca Jennings, Laura Stransky, Norbert Perrimon, Sabina Signoretti, and William Kaelin. 2019. “[HIF-Independent Synthetic Lethality Between CDK4 6 Inhibition and VHL Loss across Species](/publications/hif-independent-synthetic-lethality-between-cdk46-inhibition-and-vhl-loss-across)”. Sci Signal, 12, 601. doi:10.1126/scisignal.aay0482 Hilary Nicholson, Zeshan Tariq, Benjamin Housden, Rebecca Jennings, Laura Stransky, Norbert Perrimon, Sabina Signoretti, and William Kaelin. 2019. “[HIF-Independent Synthetic Lethality Between CDK4 6 Inhibition and VHL Loss across Species](/publications/hif-independent-synthetic-lethality-between-cdk46-inhibition-and-vhl-loss-across)”. Sci Signal, 12, 601. doi:10.1126/scisignal.aay0482 - add\_circle do\_not\_disturb\_on Abstract Inactivation of the tumor suppressor gene is the signature initiating event in clear cell renal cell carcinoma (ccRCC), the most common form of kidney cancer, and causes the accumulation of hypoxia-inducible factor 2α (HIF-2α). HIF-2α inhibitors are... [ More arrow\_circle\_right ](/publications/technology/fly-cell-based-rnai)