# Cell-based assays ## expand\_more Regardless of the technology (RNAi, CRISPR, over-expression, etc.), a good cell-based assay is the best foundation for a cell-based screen. We have [equipment](/equipment), provide [reagents](/reagents), share [protocols](/cell-based-approaches), and more to support development of high-throughput screen assays in *Drosophila* cells. Reagents, consultation, and other support is available for screens off-site. We also support [screens on-site at our facility](/on-site-screens). Assays can be done using a number of types of reagents, including reagents for knockdown or over-expression of protein-coding genes, and interrogation of miRNAs. See links below to relevant reagents, protocols, publications, and more. ![assay-hood.jpg](/sites/g/files/omnuum5366/files/fly/files/assay-hood.jpg) ## Reagents [### Drosophila cultured cell lines ](/drosophila-cultured-cell-lines) [### Cell RNAi libraries ](/fly-cell-rnai-libraries) [### RNAi rescue reagents ](/fly-rnai-rescue) ## Protocols [### Cell-based assays ](/cell-based-approaches) [### Primary cell isolation and screening ](/fly-primary-cells) [### Stable cell lines ](/stable-fly-cell-lines) ## News [### New cell lines & new understandings using cutting-edge techniques ](/news/new-cell-lines-new-understandings-using-cutting-edge-techniques) February 02, 2024 As a facility that supports large-scale screens in Drosophila and other insect cell lines, we get excited about reports of new Drosophila cell lines and related info. We'd like to highlight two recent papers. One report, a collaboration between Amanda... ![Image of green fluorescence in GFP-tagged Drosophila cultured cells](/sites/g/files/omnuum5366/files/styles/hwp_16_9__480x270/public/fly/files/drosophilacells-liveimages-gfpknockin-orip-2020-01-17_1.jpg?itok=q5-wA9BX) [### So you want to do a CRISPR pooled screen in insect cells? You can! Here's how ](/news/how-to-screen) May 12, 2022 At the DRSC-BTRR, we've been doing a lot of pooled-format CRISPR knockout screens in Drosophila cells. We're finding the results to be robust and reproducible. And best of all, the results have been informative, providing insights into diverse areas of... ![Decorative cartoon drawn with BioRender depicting DRSC-BTRR technology concepts](/sites/g/files/omnuum5366/files/styles/hwp_16_9__480x270/public/fly/files/drsc-btrr_499-350.jpeg?itok=kyu_pmTs) [### Read the DRSC/TRiP Report to the Fly Board 2022 ](/news/read-drsctrip-report-fly-board-2022) March 31, 2022 Below we share with the community at large the report we provided to the Fly Board in advance of the 2022 Fly Meeting. As always, your feedback on our technologies and services is welcome. We are here to help. DRSC/TRiP Functional Genomics Resources at... ![Decorative cartoon drawn with BioRender depicting DRSC-BTRR technology concepts](/sites/g/files/omnuum5366/files/styles/hwp_16_9__480x270/public/fly/files/drsc-btrr_499-350.jpeg?itok=kyu_pmTs) [### DRSC/TRiP and DRSC-BTRR Office Hours ](/OfficeHours2021) September 13, 2021 New this fall: Online office hours! Do you have questions about modifying Drosophila cell lines with CRISPR or performing large-scale cell screens? Questions about in vivo RNAi with TRiP fly stocks or CRISPR knockout or activation with our sgRNA fly... ![Graphical image of tissue culture, fly pushing, and computer, and the team of people who work with them](/sites/g/files/omnuum5366/files/styles/hwp_16_9__480x270/public/fly/files/team.jpeg?itok=NLIA2Ora) [### DRSC-Biomedical Technology Research Resource ](/news/drsc-biomedical-technology-research-resource) October 21, 2019 We are pleased to announce that we have been funded by NIH NIGMS to form the Drosophila Research & Screening Center-Biomedical Technology Research Resource (DRSC-BTRR). The P41-funded DRSC-BTRR (N. Perrimon, PI; S. Mohr, Co-I) builds upon and extends past... ![Graphical image of tissue culture, fly pushing, and computer, and the team of people who work with them](/sites/g/files/omnuum5366/files/styles/hwp_16_9__480x270/public/fly/files/team.jpeg?itok=NLIA2Ora) [### Drosophila cell screen with DRSC reagent library contributes to identification of new therapeutic target for renal cancer ](/news/drsc-libraries-contribute-identification-new-therapeutic-target-renal-cancer) October 07, 2019 We here at the DRSC/TRiP are thrilled to see this study from Hilary Nicholson et al. published in Science Signaling. The study provides a great example of how screens in Drosophila cultured cells can be used as part of a cross-species platform aimed at... ![Photo of 384-well assay plates](/sites/g/files/omnuum5366/files/styles/hwp_16_9__480x270/public/fly/files/img_7618.jpg?itok=FtUW2sKO) ## Contact Us Please [contact us](/contact) for any questions. ## Publications Download 114 citations download- [BibTeX](/bibcite/export?pager_style=standard_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114111&&&format=bibtex) - [EndNote X3 XML](/bibcite/export?pager_style=standard_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114111&&&format=endnote8) - [EndNote 7 XML](/bibcite/export?pager_style=standard_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114111&&&format=endnote7) - [Endnote tagged](/bibcite/export?pager_style=standard_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114111&&&format=tagged) - [Marc](/bibcite/export?pager_style=standard_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114111&&&format=marc) - [PubMedId](/bibcite/export?pager_style=standard_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114111&&&format=pubmed_id) - [RIS](/bibcite/export?pager_style=standard_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114111&&&format=ris) ### 2024 Enzo Mameli, George-Rafael Samantsidis, Raghuvir Viswanatha, Hyeogsun Kwon, David R Hall, Matthew Butnaru, Yanhui Hu, Stephanie E Mohr, Norbert Perrimon, and Ryan C Smith. 2024. “[A Genome-Wide CRISPR Screen in Mosquito Cells Identifies Essential Genes and Required Components of Clodronate Liposome Function.](/publication/genome-wide-crispr-screen-mosquito-cells-identifies-essential-genes-and-required)”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.24.614595 Enzo Mameli, George-Rafael Samantsidis, Raghuvir Viswanatha, Hyeogsun Kwon, David R Hall, Matthew Butnaru, Yanhui Hu, Stephanie E Mohr, Norbert Perrimon, and Ryan C Smith. 2024. “[A Genome-Wide CRISPR Screen in Mosquito Cells Identifies Essential Genes and Required Components of Clodronate Liposome Function.](/publication/genome-wide-crispr-screen-mosquito-cells-identifies-essential-genes-and-required)”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.24.614595 - add\_circle do\_not\_disturb\_on Abstract mosquitoes are the sole vector of human malaria, the most burdensome vector-borne disease worldwide. Strategies aimed at reducing mosquito populations and limiting their ability to transmit disease show the most promise for disease control. Therefore... ### 2023 Nikki Coleman-Gosser, Yanhui Hu, Shiva Raghuvanshi, Shane Stitzinger, Weihang Chen, Arthur Luhur, Daniel Mariyappa, Molly Josifov, Andrew Zelhof, Stephanie Mohr, Norbert Perrimon, and Amanda Simcox. 2023. “[Continuous Muscle, Glial, Epithelial, Neuronal, and Hemocyte Cell Lines for Research](/publications/continuous-muscle-glial-epithelial-neuronal-and-hemocyte-cell-lines-research)”. Elife, 12. doi:10.7554/eLife.85814 Nikki Coleman-Gosser, Yanhui Hu, Shiva Raghuvanshi, Shane Stitzinger, Weihang Chen, Arthur Luhur, Daniel Mariyappa, Molly Josifov, Andrew Zelhof, Stephanie Mohr, Norbert Perrimon, and Amanda Simcox. 2023. “[Continuous Muscle, Glial, Epithelial, Neuronal, and Hemocyte Cell Lines for Research](/publications/continuous-muscle-glial-epithelial-neuronal-and-hemocyte-cell-lines-research)”. Elife, 12. doi:10.7554/eLife.85814 - add\_circle do\_not\_disturb\_on Abstract - [ picture\_as\_pdfelife-85814-v2.pdf](/sites/g/files/omnuum5366/files/fly/files/elife-85814-v2.pdf) Expression of activated Ras, Ras, provides cultured cells with a proliferation and survival advantage that simplifies the generation of continuous cell lines. Here, we used lineage-restricted Ras expression to generate continuous cell lines of muscle... - [ picture\_as\_pdfelife-85814-v2.pdf](/sites/g/files/omnuum5366/files/fly/files/elife-85814-v2.pdf) Baolong Xia, Raghuvir Viswanatha, Yanhui Hu, Stephanie Mohr, and Norbert Perrimon. 2023. “[Pooled Genome-Wide CRISPR Activation Screening for Rapamycin Resistance Genes in Cells](/publications/pooled-genome-wide-crispr-activation-screening-rapamycin-resistance-genes-cells)”. Elife, 12. doi:10.7554/eLife.85542 Baolong Xia, Raghuvir Viswanatha, Yanhui Hu, Stephanie Mohr, and Norbert Perrimon. 2023. “[Pooled Genome-Wide CRISPR Activation Screening for Rapamycin Resistance Genes in Cells](/publications/pooled-genome-wide-crispr-activation-screening-rapamycin-resistance-genes-cells)”. Elife, 12. doi:10.7554/eLife.85542 - add\_circle do\_not\_disturb\_on Abstract - [ picture\_as\_pdfelife-85542-v1.pdf](/sites/g/files/omnuum5366/files/fly/files/elife-85542-v1.pdf) Loss-of-function and gain-of-function genetic perturbations provide valuable insights into gene function. In cells, while genome-wide loss-of-function screens have been extensively used to reveal mechanisms of a variety of biological processes... - [ picture\_as\_pdfelife-85542-v1.pdf](/sites/g/files/omnuum5366/files/fly/files/elife-85542-v1.pdf) Agustin Rolandelli, Hanna Laukaitis-Yousey, Haikel Bogale, Nisha Singh, Sourabh Samaddar, Anya O’Neal, Camila Ferraz, Matthew Butnaru, Enzo Mameli, Baolong Xia, Tays Mendes, Rainer Butler, Liron Marnin, Francy Paz, Luisa Valencia, Vipin Rana, Ciaran Skerry, Utpal Pal, Stephanie Mohr, Norbert Perrimon, David Serre, and Joao Pedra. 2023. “[Tick Hemocytes Have Pleiotropic Roles in Microbial Infection and Arthropod Fitness](/publications/tick-hemocytes-have-pleiotropic-roles-microbial-infection-and-arthropod-fitness)”. BioRxiv, Pp. 2023.08.31.555785 Agustin Rolandelli, Hanna Laukaitis-Yousey, Haikel Bogale, Nisha Singh, Sourabh Samaddar, Anya O’Neal, Camila Ferraz, Matthew Butnaru, Enzo Mameli, Baolong Xia, Tays Mendes, Rainer Butler, Liron Marnin, Francy Paz, Luisa Valencia, Vipin Rana, Ciaran Skerry, Utpal Pal, Stephanie Mohr, Norbert Perrimon, David Serre, and Joao Pedra. 2023. “[Tick Hemocytes Have Pleiotropic Roles in Microbial Infection and Arthropod Fitness](/publications/tick-hemocytes-have-pleiotropic-roles-microbial-infection-and-arthropod-fitness)”. BioRxiv, Pp. 2023.08.31.555785 - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](http://biorxiv.org/content/early/2023/09/03/2023.08.31.555785.abstract) - [ picture\_as\_pdf2023.08.31.555785v1.full\_...](/sites/g/files/omnuum5366/files/fly/files/2023.08.31.555785v1.full_.pdf) Uncovering the complexity of systems in non-model organisms is critical for understanding arthropod immunology. Prior efforts have mostly focused on Dipteran insects, which only account for a subset of existing arthropod species in nature. Here, we... - [ descriptionPublisher's Version](http://biorxiv.org/content/early/2023/09/03/2023.08.31.555785.abstract) - [ picture\_as\_pdf2023.08.31.555785v1.full\_...](/sites/g/files/omnuum5366/files/fly/files/2023.08.31.555785v1.full_.pdf) ### 2022 Ying Xu, Raghuvir Viswanatha, Oleg Sitsel, Daniel Roderer, Haifang Zhao, Christopher Ashwood, Cecilia Voelcker, Songhai Tian, Stefan Raunser, Norbert Perrimon, and Min Dong. 2022. “[CRISPR Screens in Drosophila Cells Identify Vsg As a Tc Toxin Receptor](/publications/crispr-screens-drosophila-cells-identify-vsg-tc-toxin-receptor)”. Nature, 610, 7931, Pp. 349-55. doi:10.1038/s41586-022-05250-7 Ying Xu, Raghuvir Viswanatha, Oleg Sitsel, Daniel Roderer, Haifang Zhao, Christopher Ashwood, Cecilia Voelcker, Songhai Tian, Stefan Raunser, Norbert Perrimon, and Min Dong. 2022. “[CRISPR Screens in Drosophila Cells Identify Vsg As a Tc Toxin Receptor](/publications/crispr-screens-drosophila-cells-identify-vsg-tc-toxin-receptor)”. Nature, 610, 7931, Pp. 349-55. doi:10.1038/s41586-022-05250-7 - add\_circle do\_not\_disturb\_on Abstract Entomopathogenic nematodes are widely used as biopesticides1,2. Their insecticidal activity depends on symbiotic bacteria such as Photorhabdus luminescens, which produces toxin complex (Tc) toxins as major virulence factors3-6. No protein receptors are... Hans Dalton, Raghuvir Viswanatha, Roderick Brathwaite, Jae Sophia Zuno, Alexys Berman, Rebekah Rushforth, Stephanie Mohr, Norbert Perrimon, and Clement Chow. 2022. “[A Genome-Wide CRISPR Screen Identifies DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress](/publications/genome-wide-crispr-screen-identifies-dpm1-modifier-dpagt1-deficiency-and-er-stress)”. PLoS Genet, 18, 9, Pp. e1010430. doi:10.1371/journal.pgen.1010430 Hans Dalton, Raghuvir Viswanatha, Roderick Brathwaite, Jae Sophia Zuno, Alexys Berman, Rebekah Rushforth, Stephanie Mohr, Norbert Perrimon, and Clement Chow. 2022. “[A Genome-Wide CRISPR Screen Identifies DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress](/publications/genome-wide-crispr-screen-identifies-dpm1-modifier-dpagt1-deficiency-and-er-stress)”. PLoS Genet, 18, 9, Pp. e1010430. doi:10.1371/journal.pgen.1010430 - add\_circle do\_not\_disturb\_on Abstract Partial loss-of-function mutations in glycosylation pathways underlie a set of rare diseases called Congenital Disorders of Glycosylation (CDGs). In particular, DPAGT1-CDG is caused by mutations in the gene encoding the first step in N-glycosylation... - Previous page chevron\_left - [1](?page=0 "Current page") - [2](?page=1 "Go to page 2") - [3](?page=2 "Go to page 3") - [4](?page=3 "Go to page 4") - [5](?page=4 "Go to page 5") - [6](?page=5 "Go to page 6") - [7](?page=6 "Go to page 7") - [8](?page=7 "Go to page 8") - [9](?page=8 "Go to page 9") - [ Last page 19 ](?page=18 "Go to last page") - [ Next page chevron\_right ](?page=1 "Go to next page") [ More arrow\_circle\_right ](/publications/technology/cell-based-assays)