# DRSC/TRiP Functional Genomics Resources & DRSC-BTRR ### DRSC The DRSC supports *Drosophila* cell screens using RNAi or CRISPR, generates new cell lines, and applies CRISPR screen tech to mosquito and other arthropod cell lines. ![GFP-tagged and newly isolated Drosophila cell lines generated in the project funded by R24 OD019847 (to Perrimon, Mohr, and Simcox), showing GFP localized to specific organelles and example tissue-type-specific cell lines such as a muscle-like cell line](/sites/g/files/omnuum5366/files/styles/hwp_16_9__480x270/public/2024-10/Fly_Hero_DRSC_cells.png?h=74011b40&itok=QSip11R9) ### TRiP The TRiP generates RNAi and CRISPR fly stocks for gene knockdown, knockout, and over-expression, and innovates in related areas of new *in vivo* resource development. ![Top of a vial of Drosophila showning adult flies; the TRiP has generated many transgenic Drosophila fly stocks for knockdown, knockout, binary expression, and other uses](/sites/g/files/omnuum5366/files/styles/hwp_16_9__480x270/public/2024-10/Fly_Hero_TRiP_flies.jpg?h=e316b71b&itok=nfEsbI2X) ### Online Tools Our bioinformatics suite includes tools for identifying genes, orthologs, and reagents; analyzing, visualizing, and mining datasets; and more. ![Screenshot of a grid layout of icons and links to our suite of online tools, used to demonstrate that we have a large suite of available online tools](/sites/g/files/omnuum5366/files/styles/hwp_16_9__480x270/public/2024-10/Fly_Hero_Tools_toolsgrid.png?h=dfde4a26&itok=laNac03V) ### **Please Note: This website is now considered a legacy website and is not regularly updated.** ###### **We invite you to** [**view our new Arthropod Cell Screening Facility (ACSF) website**](https://acsf.hms.harvard.edu/) **for information about current initiatives and goals, current publication lists, and more. We will retain this site as long as feasible so that the community continues to have access to DRSC protocols, TRiP info, and more.** The **Drosophila RNAi Screening Center (DRSC) & Transgenic RNAi Project (TRiP)-Functional Genomics Resources (DRSC/TRiP-FGR)** at Harvard Medical School, also known as the **Drosophila Research & Screening Center-Biomedical Technology Research Resource (DRSC-BTRR),** has been developing technology and resources for the *Drosophila* and broader research community since 2004. ### DRSC quick links - [CRISPR cell screening overview](https://fgr.hms.harvard.edu/news/how-to-screen) - [List of KO and fluorescence-tagged cell lines](https://fgr.hms.harvard.edu/crispr-modified-cell-lines) - DRSC dsRNA [reagents for cell RNAi](https://fgr.hms.harvard.edu/drsc-focused-sub-libraries) - Report on [tissue-specific cell lines](https://elifesciences.org/articles/85814) - Report on [mosquito cell screens](https://www.biorxiv.org/content/10.1101/2024.09.24.614595v1) - Order cell lines at [DGRC](https://dgrc.bio.indiana.edu/cells/Catalog) ### TRiP quick links - [TRiP fly stock and vector information](https://fgr.hms.harvard.edu/trip-in-vivo-fly-rnai) - TRiP [CRISPR knockout & over-expression fly stocks](https://fgr.hms.harvard.edu/crispr-fly-stocks-and-vectors) - TRiP [CRISPR "Toolbox" fly stock collection at BDSC](https://bdsc.indiana.edu/stocks/genome_editing/triptoolbox_grna.html) - Report on [new LexA & QF2 system flies](https://elifesciences.org/articles/94073) - Report on [new split-GAL4 flies](https://www.biorxiv.org/content/10.1101/2024.10.10.617664v1) - Order TRiP stocks at [BDSC](https://bdsc.indiana.edu/) ### Online Tools quick links - Ortholog search at [DIOPT](https://www.flyrnai.org/diopt) - Gene set enrichment analyses at [PANGEA](https://www.flyrnai.org/tools/pangea/) - Known & predicted PPIs at [MIST](https://fgrtools.hms.harvard.edu/MIST/) & [FlyPredictome](https://www.flyrnai.org/tools/fly_predictome/web/) - sgRNA designs for mosquitos and ticks at [GuideXpress](https://www.flyrnai.org/tools/fly2mosquito/web/) - Our [latest *NAR* *Database Issue* report](https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae917/7831080) - [View our full suite of online tools](https://fgr.hms.harvard.edu/tools) ## Recent News [### DIOPT online ortholog search tool updated ](/news/2026/01/diopt-online-ortholog-search-tool-updated) January 07, 2026 We recently updated our DIOPT online ortholog search tool. Notable changes include addition of predictions for the black-legged tick, Ixodes scapularis, and removal of an outdated ortholog prediction set. Please see the attached PDF for a visual summary... description [### PANGEA online tool updated ](/news/2026/01/pangea-online-tool-updated) January 07, 2026 We recently updated our PANGEA gene set enrichment analysis tool. The tool now supports gene set enrichment analysis for the malaria mosquito Anopheles gambiae and the black-legged tick Ixodes scapularis. In addition, we have added new gene sets for gene... description [### Video featuring in vivo projects and highlighting ways to connect with our group ](/news/video-featuring-vivo-projects-and-highlighing-ways-connect-our-group) June 20, 2024 We made a (very short) movie! Learn about some of our in vivo projects and ways to collaborate with our group. Click below to start the 2-minute movie playing in your browser window: cmrdm_drsc_final.mp4 description [ More arrow\_circle\_right ](/news) [ More Events arrow\_circle\_right ](/calendar) ## Recent Preprints & Publications Download 221 citations download- [BibTeX](/bibcite/export?pager_style=standard_pager&number_of_items=3&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_organism%5D&taxonomy_filters%5Bfield_hwp_c_publicationtype1234%5D&taxonomy_filters%5Bfield_hwp_c_technology%5D&taxonomy_filters%5Bfield_hwp_c_topics12%5D&&&format=bibtex) - [EndNote X3 XML](/bibcite/export?pager_style=standard_pager&number_of_items=3&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_organism%5D&taxonomy_filters%5Bfield_hwp_c_publicationtype1234%5D&taxonomy_filters%5Bfield_hwp_c_technology%5D&taxonomy_filters%5Bfield_hwp_c_topics12%5D&&&format=endnote8) - [EndNote 7 XML](/bibcite/export?pager_style=standard_pager&number_of_items=3&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_organism%5D&taxonomy_filters%5Bfield_hwp_c_publicationtype1234%5D&taxonomy_filters%5Bfield_hwp_c_technology%5D&taxonomy_filters%5Bfield_hwp_c_topics12%5D&&&format=endnote7) - [Endnote tagged](/bibcite/export?pager_style=standard_pager&number_of_items=3&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_organism%5D&taxonomy_filters%5Bfield_hwp_c_publicationtype1234%5D&taxonomy_filters%5Bfield_hwp_c_technology%5D&taxonomy_filters%5Bfield_hwp_c_topics12%5D&&&format=tagged) - [Marc](/bibcite/export?pager_style=standard_pager&number_of_items=3&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_organism%5D&taxonomy_filters%5Bfield_hwp_c_publicationtype1234%5D&taxonomy_filters%5Bfield_hwp_c_technology%5D&taxonomy_filters%5Bfield_hwp_c_topics12%5D&&&format=marc) - [PubMedId](/bibcite/export?pager_style=standard_pager&number_of_items=3&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_organism%5D&taxonomy_filters%5Bfield_hwp_c_publicationtype1234%5D&taxonomy_filters%5Bfield_hwp_c_technology%5D&taxonomy_filters%5Bfield_hwp_c_topics12%5D&&&format=pubmed_id) - [RIS](/bibcite/export?pager_style=standard_pager&number_of_items=3&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_organism%5D&taxonomy_filters%5Bfield_hwp_c_publicationtype1234%5D&taxonomy_filters%5Bfield_hwp_c_technology%5D&taxonomy_filters%5Bfield_hwp_c_topics12%5D&&&format=ris) ### 2026 Matthew Butnaru, William McKenna, Srishti Goswami, Alejandra Wu-Chuang, Enzo Mameli, Abigail Wilcox, Leopoldine Quennesson, Ah-Ram Kim, Austin Veal, Weihang Chen, Hugo Verzone, Elizabeth A Lane, Hanna J Laukaitis-Yousey, Chad Araneo, Nisha Singh, Joao Pedra, Yanhui Hu, Raghuvir Viswanatha, Norbert Perrimon, and Stephanie E Mohr. 2026. “[Genome-Wide CRISPR Knockout Cell Screening Platform for the Disease Vector Tick Species Ixodes Scapularis](/publication/genome-wide-crispr-knockout-cell-screening-platform-disease-vector-tick-species-ixodes)”. BioRxiv. doi:10.64898/2026.05.05.721418 Matthew Butnaru, William McKenna, Srishti Goswami, Alejandra Wu-Chuang, Enzo Mameli, Abigail Wilcox, Leopoldine Quennesson, Ah-Ram Kim, Austin Veal, Weihang Chen, Hugo Verzone, Elizabeth A Lane, Hanna J Laukaitis-Yousey, Chad Araneo, Nisha Singh, Joao Pedra, Yanhui Hu, Raghuvir Viswanatha, Norbert Perrimon, and Stephanie E Mohr. 2026. “[Genome-Wide CRISPR Knockout Cell Screening Platform for the Disease Vector Tick Species Ixodes Scapularis](/publication/genome-wide-crispr-knockout-cell-screening-platform-disease-vector-tick-species-ixodes)”. BioRxiv. doi:10.64898/2026.05.05.721418 - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](https://www.biorxiv.org/content/early/2026/05/07/2026.05.05.721418) The black legged tick, Ixodes scapularis, is a vector of the bacterium that causes Lyme disease and several other illnesses, including anaplasmosis, babesiosis, and tick-borne encephalitis. Although high-quality genome annotations are available for I... - [ descriptionPublisher's Version](https://www.biorxiv.org/content/early/2026/05/07/2026.05.05.721418) ### 2025 Baolong Xia, Ah-Ram Kim, Feimei Liu, Myeonghoon Han, Emily Stoneburner, Stephanie Makdissi, Francesca Di Cara, Stephanie E Mohr, Aaron Ring, and Norbert Perrimon. 2025. “[Phage-Displayed Synthetic Library and Screening Platform for Nanobody Discovery.](/publication/phage-displayed-synthetic-library-and-screening-platform-nanobody-discovery)”. ELife, 14. doi:10.7554/eLife.105887 Baolong Xia, Ah-Ram Kim, Feimei Liu, Myeonghoon Han, Emily Stoneburner, Stephanie Makdissi, Francesca Di Cara, Stephanie E Mohr, Aaron Ring, and Norbert Perrimon. 2025. “[Phage-Displayed Synthetic Library and Screening Platform for Nanobody Discovery.](/publication/phage-displayed-synthetic-library-and-screening-platform-nanobody-discovery)”. ELife, 14. doi:10.7554/eLife.105887 - add\_circle do\_not\_disturb\_on Abstract Nanobodies, single-domain antibodies derived from camelid heavy-chain antibodies, are known for their high affinity, stability, and small size, which make them useful in biological research and therapeutic applications. However, traditional nanobody... Justin A Bosch, Pierre Michel Jean Beltran, Cooper Cavers, James Thai LaGraff, Randy Melanson, Ankita Singh, Weihang Chen, Yanhui Hu, Sudhir Gopal Tattikota, Ying Liu, Yousuf Hashmi, John M Asara, Tess Branon, Alice Y Ting, Steven A Carr, and Norbert Perrimon. 2025. “[Multi-Omic Mapping of Protein Secretomes Reveals Tissue-Specific Origins and Inter-Organ Trafficking.](/publication/multi-omic-mapping-protein-secretomes-reveals-tissue-specific-origins-and-inter-organ)”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2025.07.09.659702 Justin A Bosch, Pierre Michel Jean Beltran, Cooper Cavers, James Thai LaGraff, Randy Melanson, Ankita Singh, Weihang Chen, Yanhui Hu, Sudhir Gopal Tattikota, Ying Liu, Yousuf Hashmi, John M Asara, Tess Branon, Alice Y Ting, Steven A Carr, and Norbert Perrimon. 2025. “[Multi-Omic Mapping of Protein Secretomes Reveals Tissue-Specific Origins and Inter-Organ Trafficking.](/publication/multi-omic-mapping-protein-secretomes-reveals-tissue-specific-origins-and-inter-organ)”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2025.07.09.659702 - add\_circle do\_not\_disturb\_on Abstract Secreted proteins regulate many aspects of animal biology and are attractive targets for biomarkers and therapeutics. However, comprehensively identifying the "secretome", along with their tissues of origin, remains extremely challenging. To address this... - Previous page chevron\_left - [1](?page=0 "Current page") - [2](?page=1 "Go to page 2") - [3](?page=2 "Go to page 3") - [4](?page=3 "Go to page 4") - [5](?page=4 "Go to page 5") - [6](?page=5 "Go to page 6") - [7](?page=6 "Go to page 7") - [8](?page=7 "Go to page 8") - [9](?page=8 "Go to page 9") - [ Last page 74 ](?page=73 "Go to last page") - [ Next page chevron\_right ](?page=1 "Go to next page") [ View All Citations arrow\_circle\_right ](/publications) [### DRSC-BTRR ](/drsc-btrr)Technology R&D at the DRSC-BTRR ![Decorative cartoon drawn with BioRender depicting DRSC-BTRR technology concepts](/sites/g/files/omnuum5366/files/styles/hwp_16_9__480x270/public/fly/files/drsc-btrr_499-350.jpeg?itok=kyu_pmTs) [### in vivo RNAi (TRiP) ](/fly-in-vivo-rnai)Drosophila in vivo RNAi ![in vivo](/sites/g/files/omnuum5366/files/styles/hwp_16_9__480x270/public/fly/files/invivo-tile.jpg?itok=ZEK1O34D) [### online tools ](/tools)online software and databases ![Screenshot image of our Online Tools Overview page](/sites/g/files/omnuum5366/files/styles/hwp_16_9__480x270/public/fly/files/grid_fgr-home.png?itok=RYdQnNRO) [### cell RNAi (DRSC) ](/fly-cell-based-rnai)Drosophila cell-based RNAi resources ![Cell RNAi](/sites/g/files/omnuum5366/files/styles/hwp_16_9__480x270/public/fly/files/cell-rnai-tile.jpg?itok=QobndeqL) [### CRISPR technologies ](/technologies)Drosophila cell and in vivo CRISPR ![CRISPR Technologies](/sites/g/files/omnuum5366/files/styles/hwp_16_9__480x270/public/fly/files/crispr-tile.jpg?itok=jfRxTVhJ)