#  Cell-based RNAi 

 



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 We recommend the following workflow.

- dsRNA synthesis from DRSC reagent collection templates (PCR amplification, in vitro transcription, normalization)
- incubation of cells with RNAi and cell-based assay

 We provide protocols for 6-well and 384-well plates, with additional tips on quantities for 96-well plates (see 384-well plate protocol). Experiments may be done in other size plates, just scale up or down, and test the assay (some adjustments might be needed).

 Please note that a number of factors will influence RNAi knockdown, including

- reagent design
- reagent quality/purity
- reagent amount
- cell type
- cell density
- cell passage number (tip: don't let cells overgrow or passage them for a long time prior to the experiment--they might stop responding to RNAi reagents)

 See menu on the left-hand side of this page for links to specific protocols.

 Feel free to [contact us](/contact) with questions about cell-based RNAi in small or large-scale studies.