# DIOPT Version 8 ## expand\_more Webiste: [https://www.flyrnai.org/cgi-bin/DRSC\_orthologs\_v08.pl](https://www.flyrnai.org/cgi-bin/DRSC_orthologs_v08.pl) [Version 9 information](/diopt-documentation) ### About DIOPT The identification of orthologs is commonly used for bioinformatics activities such as data mining and establishing models for human diseases. Moreover, our group notes that researchers analyzing the results of screens performed at the Drosophila RNAi Screening Center (DRSC) frequently wish to identify mammalian orthologs of the fly genes that were "hits" (positive results) in their screens. In helping DRSC screeners to identify orthologs using existing tools and algorithms, we recognized a need for a user-friendly approach to viewing and comparing ortholog predictions obtained using different tools and algorithms. This was our motivation in developing DIOPT. To facilitate identification of orthologs specifically of human disease-associated genes, we further developed DIOPT-DIST. Information about our approaches to development of both tools is summarized below. ### The DIOPT Approach Many tools have emerged to meet the need to identify orthologs. However, low coverage and heterogeneity of these tools present an obstacle to scientists who want to identify a one or a few highest-confidence orthologs for a given gene of interest or conversely, want to cast a wide net and follow up on all possible orthologs of a gene. Our goal is to provide an easy-to-use resource that facilitates summary, comparison and access to various sources of ortholog predictions. DIOPT integrates human, mouse, fly, worm, zebrafish and yeast ortholog predictions made by Ensembl Compara, HomoloGene, Inparanoid, Isobase, OMA, orthoMCL, Phylome, RoundUp, and TreeFam. DIOPT lets users find ortholog pairs for a specified gene or genes identified by one, many or all of these published approaches. This provides a streamlined method for integration, comparison and access to orthology predictions originating from algorithms based on sequence homology, phylogenetic trees, and functional similarity. DIOPT calculates a simple score indicating the number of tools that support a given orthologous gene-pair relationship, as well as a weighted score based on functional assessment using high quality GO molecular function annotation of all fly-human orthologous pairs predicted by each tool. Differences in the algorithms used by each tool to predict orthologous relationship is one source of difference in the set of predictions made by one tool versus another. However, we also note that some of these differences might be attributable to use of different genome annotation releases used by some tools versus others, and that not all tools cover all of the species that we include in the DIOPT tool (see Tables 1,2 and 3). DIOPT also displays protein and domain alignments, including percent amino acid identity, for predicted ortholog pairs. These should help you to identify the most appropriate matches among multiple possible orthologs. The following summary figures and tables help to explain our approach and summarize the tools and algorithms included in DIOPT. Sort**Figure 1: Summary of the DIOPT approach to integration of results from multiple ortholog prediction tools and algorithms.** In green, tools based on sequence alignment. In purple, tools based on evolutionary relationships. In orange, a tool that incorporates protein-protein interaction network data into ortholog predictions. ### Table 1: Summary Information and Publications for the Tools Integrated in DIOPT Sort**Prediction Method** **Source** **Prediction Algorithm** **Coverage** **PMID** [Compara](http://ensembl.org/info/docs/api/compara/index.html) Ensembl Phylogenetic approach 200 species (vs 96) [19029536](http://www.ncbi.nlm.nih.gov/pubmed/19029536) [Homologene](http://www.ncbi.nlm.nih.gov/homologene) NCBI Combination of BBH*, tree and synteny 21 species (vs. 68) [11125071](http://www.ncbi.nlm.nih.gov/pubmed/11125071) [Inparanoid ](http://inparanoid.sbc.su.se/cgi-bin/index.cgi) Stockholm University, Sweden BBH* approach to identify orthologs and in-paralogs 273 species (vs. 8) [11743721](http://www.ncbi.nlm.nih.gov/pubmed/11743721) [25429972](http://www.ncbi.nlm.nih.gov/pubmed/25429972) [Isobase](http://groups.csail.mit.edu/cb/mna/isobase/) MIT Sequence and PPI* network alignments 5 species (vs.2, Nov. 2014) [21177658](http://www.ncbi.nlm.nih.gov/pubmed/21177658) [OMA](http://omabrowser.org/cgi-bin/gateway.pl) CBRG, ETH Zurich BBH*, global sequence alignments 2198 species (Jun 2018 release) [17545180](http://www.ncbi.nlm.nih.gov/pubmed/17545180) [OrthoDB](http://cegg.unige.ch/orthodb6) University of Geneva Phylogenetic approach >5000 species (vs.10) [20972218](http://www.ncbi.nlm.nih.gov/pubmed/20972218) [25428351](http://www.ncbi.nlm.nih.gov/pubmed/25428351) [orthoMCL](http://www.orthomcl.org/cgi-bin/OrthoMclWeb.cgi) University of Pennsylvania RSD, modified BBH* (normalized) with Markov clustering 150 species (vs. 5) [12952885](http://www.ncbi.nlm.nih.gov/pubmed/12952885) [Phylome](http://phylomedb.org/) Centre for Genomic Regulation (CRG), Spain Reconstruction of evolutionary histories of all genes in a genome, also known as phylome. 1059 species,120 Phylomes (vs. 4) QfO [17962297](http://www.ncbi.nlm.nih.gov/pubmed/17962297) [24275491](http://www.ncbi.nlm.nih.gov/pubmed/24275491) [RoundUp](http://www.ncbi.nlm.nih.gov/pubmed/22247275) Harvard Medical School RSD*, modified BBH* 2044 species (Apr 2013) [16777906](http://www.ncbi.nlm.nih.gov/pubmed/16777906) [TreeFam](http://www.treefam.org/) Wellcome Trust Sanger Institute Manually curated based on trees 109 species (vs. 9) [16381935](http://www.ncbi.nlm.nih.gov/pubmed/16381935) [24194607](http://www.ncbi.nlm.nih.gov/pubmed/24194607) [Panther ](http://pantherdb.org/) University of Southern California Phylogenetic approach 132 species (vs. 14.1) [26578592 ](http://www.ncbi.nlm.nih.gov/pubmed/26578592) [HGNC ](http://www.genenames.org/) European Bioinformatics Institute (EMBL-EBI) Manually curated 3 species (July 2019) [ZFIN ](http://zfin.org/ZFIN) Zebrafish Model Organism Database Sequence similarity analysis and manual curation 4 species (July 2019) [eggNOG](http://eggnogdb.embl.de) Embl, Germany Graph-based algorithms 5090 species (vs. 5.0) [26582926 ](http:/www.ncbi.nlm.nih.gov/pubmed/26582926) [OrthoFinder](http://www.stevekellylab.com/software/orthofinder) University of Oxford Graph-based algorithms Run using protein sequence of refseq vs94 [26578592](http:/www.ncbi.nlm.nih.gov/pubmed/26578592) [OrthoInspector](http://lbgi.igbmc.fr/orthoinspector/OrthoInspector) Institut de Génétique et de Biologie Moléculaire et Cellulaire A novel orthogroup inference algorithm that solves a previously undetected gene length bias in orthogroup inference, resulting in significant improvements in accuracy. QFO 2018 [21219603](http:/www.ncbi.nlm.nih.gov/pubmed/21219603) [Hieranoid](http://hieranoid.sbc.su.se) Stockholm Bioinformatics Center The OrthoInspector algorithm is divided into three main steps. First, the results of a Blast all-versus-all (proteomes are blasted against each other) is provided by the user and is parsed to find all the Blast best hits for each protein and to create the groups of inparalogs. Second, the inparalog groups for each organism are compared in a pairwise fashion to define potential orthologs and/or in-paralogs. Third, best hits that contradict the potential orthology between entities are detected. QFO 2018 [27742821](http:/www.ncbi.nlm.nih.gov/pubmed/27742821) BBH, Best Blast Hits RSD, Reciprocal Smallest Distance PPI, Protein-Protein Interactions ### Table 2A: Genome Release Information for the Tools Integrated in DIOPT Sort Worm Fish Fly Human Mouse Yeast Fission Yeast Frog Rat Thale cress Compara WBcel235 GRCz11 BDGP6.22 GRCh38 GRCm38 R64-1-1 NA JGI 4.2 Rnor_6.0 Homologene WS195 Zv9 FlyBase r5.48 GRCh38 GRCm38.p2 R64-1-1 ASM294v2 Xtropicalis_v7 Rnor_5.0 TAIR10 OMA Ensembl 86; WBcel235; 14-SEP-2016 Ensembl 90; GRCz10 Ensembl 90; BDGP6 Ensembl 86; GRCh38; 13-SEP-2016 Ensembl 86; GRCm38; 13-SEP-2016 Ensembl 73; EF4; 23-AUG-2013 Ensembl Fungi 22; ASM294v2; 17-MAR-2014 Ensembl 73; JGI_4.2; 23-AUG-2013 Ensembl 83; Rnor_6.0; 28-NOV-2015 Ensembl Plants 20; TAIR10; 2-SEP-2013 Inparanoid UniProt Nov 2013 UniProt Nov 2013 UniProt Nov 2013 UniProt Nov 2013 UniProt Nov 2013 UniProt Nov 2013 UniProt Nov 2013 UniProt Nov 2013 UniProt Nov 2013 UniProt Nov 2013 Isobase Ensembl v59 NA Ensembl v59 Ensembl v59 Ensembl v59 Ensembl v59 NA NA NA NA orthoMCL WS206 Zv8.56 BDGP5.13.56 GRCh37.56 NCBI v37.56 FungiDB GenBank NA Ensembl v53 GenBank orthoDB Refseq Refseq Refseq Refseq Refseq Refseq Refseq Refseq Refseq Refseq RoundUp UniProt Apr 2013 UniProt Apr 2013 UniProt Apr 2013 UniProt Apr 2013 UniProt Apr 2013 UniProt Apr 2013 UniProt Apr 2013 UniProt Apr 2013 NA NA TreeFam Ensembl v69 Ensembl v69 Ensembl v69 Ensembl v69 Ensembl v69 Ensembl v69 Ensembl v69 Ensembl v69 Ensembl v69 NA Panther UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 Phylome UniProt UniProt UniProt UniProt UniProt UniProt UniProt UniProt UniProt UniProt HGNC NA NA NA HGNC July 2019 HGNC July 2019 NA NA NA HGNC July 2019 NA ZFIN NA ZFIN July 2019 ZFIN July 2019 ZFIN July 2019 ZFIN July 2019 NA NA NA NA NA eggNOG Ensembl/Refseq Ensembl/Refseq Ensembl/Refseq Ensembl/Refseq Ensembl/Refseq Ensembl/Refseq NA Ensembl/Refseq Ensembl/Refseq NA OrthoFinder RefSeq94 RefSeq94 RefSeq94 RefSeq94 RefSeq94 RefSeq94 RefSeq94 RefSeq94 RefSeq94 RefSeq94 OrthoInspector UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 Hieranoid UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 UniProt_QFO2018 #### Table 2B: Additional Information About Genome Releases SortOther ResourceVersionWormBase release272 FlyBase release6.28 RefSeq release94 EntrezGene 11-July-2019 ### **Table 3. Maximum DIOPT score for each orthologous relationship** SortOrthologous Relationship Max score Type Relevant Tools Orthologous Relationship Max score Type Relevant Tools baker's yeast-fish 13 ortholog Inparanoid;TreeFam;RoundUp;Phylome;Panther;orthoMCL;OrthoInspector;OMA;Homologene;Hieranoid;eggNOG;Compara;OrthoFinder; baker's yeast-fission yeast 11 ortholog OrthoInspector;RoundUp;Phylome;TreeFam;orthoMCL;OMA;Inparanoid;Homologene;Hieranoid;Panther;OrthoFinder; baker's yeast-fly 14 ortholog Phylome;RoundUp;Panther;orthoMCL;OrthoInspector;OrthoFinder;Isobase;Inparanoid;Homologene;Hieranoid;eggNOG;Compara;OMA;TreeFam; baker's yeast-frog 12 ortholog Inparanoid;RoundUp;Phylome;Panther;OrthoInspector;TreeFam;Homologene;Hieranoid;eggNOG;Compara;OrthoFinder;OMA; baker's yeast-human 14 ortholog OMA;RoundUp;Phylome;Panther;orthoMCL;OrthoFinder;TreeFam;Isobase;Inparanoid;Homologene;Hieranoid;eggNOG;Compara;OrthoInspector; baker's yeast-mouse 14 ortholog OrthoFinder;RoundUp;TreeFam;Phylome;Panther;orthoMCL;OrthoInspector;OMA;Isobase;Inparanoid;Homologene;Hieranoid;Compara;eggNOG; baker's yeast-rat 12 ortholog Inparanoid;TreeFam;Phylome;Panther;orthoMCL;OrthoInspector;OMA;Homologene;Hieranoid;eggNOG;Compara;OrthoFinder; baker's yeast-Thale cress 8 ortholog Inparanoid;Phylome;Panther;orthoMCL;OMA;Homologene;Hieranoid;OrthoInspector; baker's yeast-worm 14 ortholog OrthoFinder;RoundUp;orthoMCL;Panther;TreeFam;OrthoInspector;Isobase;Inparanoid;Homologene;Hieranoid;eggNOG;Compara;OMA;Phylome; fish-fission yeast 11 ortholog orthoMCL;Homologene;RoundUp;Phylome;TreeFam;Panther;OrthoInspector;OrthoFinder;Inparanoid;Hieranoid;OMA; fish-fly 15 ortholog OrthoInspector;Compara;TreeFam;RoundUp;Phylome;Panther;orthoMCL;ZFIN;OrthoFinder;OrthoDB;OMA;Inparanoid;Homologene;eggNOG;Hieranoid; fish-frog 13 ortholog OrthoFinder;TreeFam;RoundUp;Phylome;OrthoInspector;OrthoDB;OMA;Inparanoid;Homologene;Hieranoid;eggNOG;Compara;Panther; fish-human 15 ortholog eggNOG;OrthoInspector;TreeFam;RoundUp;ZFIN;Phylome;Panther;orthoMCL;OrthoFinder;OrthoDB;OMA;Inparanoid;Hieranoid;Compara;Homologene; fish-mouse 15 ortholog OrthoInspector;Homologene;ZFIN;TreeFam;RoundUp;Phylome;Panther;orthoMCL;OrthoDB;Compara;Inparanoid;OMA;Hieranoid;eggNOG;OrthoFinder; fish-rat 13 ortholog OrthoInspector;TreeFam;Phylome;orthoMCL;OrthoFinder;OrthoDB;Inparanoid;Homologene;Hieranoid;eggNOG;Compara;OMA;Panther; fish-Thale cress 9 ortholog OMA;Phylome;Panther;orthoMCL;OrthoDB;Inparanoid;Homologene;Hieranoid;OrthoInspector; fish-worm 14 ortholog OrthoInspector;TreeFam;RoundUp;Phylome;orthoMCL;OrthoFinder;eggNOG;Panther;Compara;OrthoDB;Hieranoid;Homologene;Inparanoid;OMA; fission yeast-fly 11 ortholog OMA;RoundUp;Phylome;Panther;TreeFam;orthoMCL;Inparanoid;Homologene;Hieranoid;OrthoInspector;OrthoFinder; fission yeast-frog 10 ortholog OrthoInspector;Phylome;RoundUp;Panther;OMA;Inparanoid;OrthoFinder;Homologene;Hieranoid;TreeFam; fission yeast-human 11 ortholog RoundUp;TreeFam;Phylome;Panther;orthoMCL;OrthoFinder;OMA;Inparanoid;Hieranoid;Homologene;OrthoInspector; fission yeast-mouse 11 ortholog OMA;TreeFam;RoundUp;Phylome;Panther;orthoMCL;Hieranoid;OrthoFinder;Inparanoid;Homologene;OrthoInspector; fission yeast-rat 10 ortholog OrthoInspector;Panther;Phylome;orthoMCL;OMA;Inparanoid;Hieranoid;Homologene;TreeFam;OrthoFinder; fission yeast-Thale cress 8 ortholog orthoMCL;Homologene;Phylome;Panther;OrthoInspector;Inparanoid;Hieranoid;OMA; fission yeast-worm 11 ortholog OrthoFinder;TreeFam;RoundUp;Phylome;Panther;Hieranoid;OrthoInspector;OMA;Inparanoid;Homologene;orthoMCL; fly-frog 13 ortholog OrthoFinder;RoundUp;TreeFam;Phylome;Panther;OrthoInspector;OrthoDB;OMA;Inparanoid;Homologene;Hieranoid;Compara;eggNOG; fly-human 16 ortholog OrthoInspector;Compara;TreeFam;User_Submission;RoundUp;Phylome;Panther;orthoMCL;OrthoFinder;Hieranoid;eggNOG;Homologene;Inparanoid;Isobase;OMA;OrthoDB; fly-mouse 15 ortholog Isobase;OrthoFinder;RoundUp;Phylome;Panther;orthoMCL;OrthoInspector;TreeFam;OMA;Inparanoid;Homologene;Hieranoid;eggNOG;Compara;OrthoDB; fly-rat 13 ortholog OrthoDB;orthoMCL;Panther;TreeFam;OrthoInspector;OrthoFinder;Inparanoid;Homologene;Hieranoid;eggNOG;Compara;Phylome;OMA; fly-Thale cress 9 ortholog OMA;Phylome;Panther;orthoMCL;OrthoDB;Inparanoid;Homologene;Hieranoid;OrthoInspector; fly-worm 15 ortholog OrthoFinder;TreeFam;RoundUp;Phylome;Panther;orthoMCL;OrthoInspector;OrthoDB;OMA;Isobase;Inparanoid;Homologene;Hieranoid;Compara;eggNOG; frog-human 13 ortholog OrthoDB;RoundUp;Phylome;Panther;OrthoFinder;OMA;Inparanoid;Homologene;Hieranoid;eggNOG;Compara;TreeFam;OrthoInspector; frog-mouse 13 ortholog OrthoFinder;TreeFam;RoundUp;Phylome;Panther;OrthoInspector;OMA;Inparanoid;Homologene;Hieranoid;eggNOG;Compara;OrthoDB; frog-rat 12 ortholog Panther;Phylome;OrthoInspector;OrthoFinder;OrthoDB;Inparanoid;Homologene;Hieranoid;eggNOG;Compara;TreeFam;OMA; frog-Thale cress 8 ortholog Homologene;Phylome;Panther;OrthoInspector;OrthoDB;Inparanoid;Hieranoid;OMA; frog-worm 13 ortholog OrthoFinder;RoundUp;TreeFam;Phylome;Panther;OrthoInspector;OrthoDB;OMA;Inparanoid;Homologene;Hieranoid;Compara;eggNOG; human-mouse 16 ortholog eggNOG;OrthoFinder;TreeFam;RoundUp;Phylome;Panther;orthoMCL;OrthoInspector;OrthoDB;OMA;Isobase;Inparanoid;Homologene;HGNC;Compara;Hieranoid; human-rat 14 ortholog OrthoFinder;HGNC;TreeFam;Phylome;Panther;orthoMCL;OrthoInspector;OMA;Inparanoid;Hieranoid;Homologene;eggNOG;Compara;OrthoDB; human-Thale cress 9 ortholog OMA;Phylome;Panther;orthoMCL;OrthoDB;Inparanoid;Homologene;Hieranoid;OrthoInspector; human-worm 15 ortholog OrthoFinder;eggNOG;RoundUp;Phylome;Panther;orthoMCL;OrthoInspector;TreeFam;OrthoDB;OMA;Isobase;Inparanoid;Hieranoid;Compara;Homologene; mouse-rat 13 ortholog Inparanoid;TreeFam;Phylome;Panther;orthoMCL;OrthoInspector;OrthoFinder;OMA;Homologene;Hieranoid;eggNOG;Compara;OrthoDB; mouse-Thale cress 9 ortholog OMA;Phylome;Panther;orthoMCL;OrthoDB;Inparanoid;Homologene;Hieranoid;OrthoInspector; mouse-worm 15 ortholog OMA;TreeFam;RoundUp;Phylome;Panther;orthoMCL;OrthoInspector;OrthoDB;Isobase;Inparanoid;Homologene;Hieranoid;eggNOG;Compara;OrthoFinder; rat-Thale cress 9 ortholog OrthoInspector;Hieranoid;Phylome;Panther;orthoMCL;OMA;Inparanoid;Homologene;OrthoDB; rat-worm 13 ortholog OrthoDB;orthoMCL;Panther;TreeFam;OrthoInspector;OrthoFinder;Inparanoid;Homologene;Hieranoid;eggNOG;Compara;Phylome;OMA; Thale cress-worm 9 ortholog OMA;Phylome;Panther;orthoMCL;OrthoDB;Inparanoid;Homologene;Hieranoid;OrthoInspector; baker's yeast-baker's yeast 9 paralog Inparanoid;RoundUp;Panther;orthoMCL;Isobase;Homologene;eggNOG;Compara;OrthoFinder; fish-fish 9 paralog OrthoDB;OrthoFinder;RoundUp;orthoMCL;Compara;Homologene;Panther;eggNOG;Inparanoid; fission yeast-fission yeast 6 paralog Homologene;Inparanoid;OrthoFinder;orthoMCL;Panther;RoundUp; fly-fly 10 paralog OrthoFinder;OrthoDB;RoundUp;Panther;Compara;Inparanoid;orthoMCL;Homologene;eggNOG;Isobase; frog-frog 7 paralog OrthoFinder;Homologene;RoundUp;Compara;eggNOG;OrthoDB;Inparanoid; human-human 11 paralog OMA;RoundUp;Panther;orthoMCL;OrthoDB;Isobase;Inparanoid;Homologene;eggNOG;Compara;OrthoFinder; mouse-mouse 12 paralog Panther;Phylome;orthoMCL;OrthoFinder;OrthoDB;Isobase;Inparanoid;Homologene;Compara;eggNOG;OMA;RoundUp; rat-rat 8 paralog Panther;Compara;eggNOG;Homologene;Inparanoid;OrthoDB;OrthoFinder;orthoMCL; Thale cress-Thale cress 5 paralog Inparanoid;OrthoDB;orthoMCL;Panther;Homologene; worm-worm 10 paralog Isobase;RoundUp;Panther;orthoMCL;OrthoDB;Inparanoid;Homologene;eggNOG;Compara;OrthoFinder; #### **Table 4. DIOPT Weights** DIOPT weights were calculated based on the mean semantic similarity of high quality GO molecular function annotation of all fly-human orthologous pairs predicted by each tool from DIOPT vs1. As such, they do not predict overall performance of an algorithm. Please go to the DIOPT publication for detailed information (). SortPrediction MethodDIOPT WeightCompara 0.93 Homologene 1 Inparanoid 1.05 Isobase 0.95 OMA 1 .01 OrthoDB 1.01 OrthoMCL 0.9 Phylome 0.91 RoundUp 1.03 TreeFam 0.96 Panther 1.1 HGNC 1.5 ZFIN 1.5 eggNOG 0.9 OrthoFinder 1 OrthoInspector 1 Hieranoid 1 ## **SCORE DISTRIBUTIONS** ### Ortholog Score Distribution [diopt\_data\_ortholog\_2019.png](/file_url/723) ### Paralog Score Distribution [diopt\_data\_paralog\_2019.png](/file_url/724) ## Version information 8.0- Aug 2019 - - Updated [Data Sources](/diopt-documentation) 7.1- Mar 2018 - - Allow user to submit missing relationships - Allow user to add feedback 7.0- Jan 2018 - - Updated [Data Sources](/diopt-documentation) - added 3 new algorithms: OrthoFinder, OrthoInspector, Hieranoid as a sources - added new species: Arabidopsis (Thale Cress) 6.0- Dec 2016 - - Updated Data Sources - added eggNOG as a source - Added paralogs 5.5- Oct 2016 - Added multi-sequence alignment from target "All" heatmap 5.4- Sept 2016 - Added target species "All" and new filter 5.3- May 2016 - Added more prediction tools (Panther, HGNC and ZFIN) 5.2.1- April 2016 - Added orthologous rank High: best score both ways AND DIOPT score >=2 Moderate: (best score forward or reverse) AND DIOPT score >=2 DIOPT score >=4 Low: all others 5.2- April 2016 - Added New Spcecies (Rattus norvegicus) 5.1.1 - December 2015 - Added Best forward and reverse columns 5.1 - November 2015 - Upgraded gene matching algorithm 5.0 - November 2015 - Upgraded data sources to version 5