# CRISPR modified cell lines ## expand\_more **CRISPR modified cell lines** We are using CRISPR gene editing technologies to generate new cell lines as part of the funded project NIH ORIP R24 OD019847 "Next-generation Drosophila cell lines to elucidate the cellular basis of human diseases" (N. Perrimon, PI; A. Simcox, Co-PI). **GFP-tagged knock-in cell lines** As part of the ORIP-funded project, we are making GFP-tagged cell lines, with an emphasis on visualization of various organelles and sub-cellular compartments. The following GFP knock-in cell lines made at the DRSC in collaboration with the Bellne lab are available for distribution by the DGRC in Bloomington, IN. Note that the parental cell line is positive for an mCherry fusion and Cas9, as the parental cell line is S2R+-MT::Cas9 ([DGRC cell catalog #268](https://dgrc.bio.indiana.edu/product/View?product=268)), which is described in Viswanatha et al. 2018 ([PubMed ID 30051818](https://www.ncbi.nlm.nih.gov/pubmed/30051818)). This parental cell line was itself derived from DRSC cell line S2R+ NPT005 ([DGRC cell catalog #229](https://dgrc.bio.indiana.edu/product/View?product=229)), which is described in Neumuller et al. 2012 ([PubMed ID 22174071](https://www.ncbi.nlm.nih.gov/pubmed/22174071)). S2R+ with GFP::Cnx99a. Ordering information: [DGRC cell catalog ID #273](https://dgrc.bio.indiana.edu/product/View?product=273) S2R+ with GFP::Rab11. Ordering information: [DGRC cell catalog ID #274](https://dgrc.bio.indiana.edu/product/View?product=274) S2R+ with GFP::Polo. Ordering information: [DGRC cell catalog ID #275](https://dgrc.bio.indiana.edu/product/View?product=275) S2R+ with GFP::Gmap (clone #4). Ordering information: [DGRC cell catalog ID #276](https://dgrc.bio.indiana.edu/product/View?product=276) S2R+ with GFP::Gmap (clone $7). Ordering information: [DGRC cell catalog ID #277](https://dgrc.bio.indiana.edu/product/View?product=277) S2R+ with GFP::Fib (clone #11). Ordering information: [DGRC cell catalog ID #278](https://dgrc.bio.indiana.edu/product/View?product=278) S2R+ with GFP::Fib (clone #12). Ordering information: [DGRC cell catalog ID #279](https://dgrc.bio.indiana.edu/product/View?product=279) S2R+ with GFP::Golgin. Ordering information: [DGRC cell catalog ID #280](https://dgrc.bio.indiana.edu/product/View?product=280) S2R+ with GFP::Arl8. Ordering information: [DGRC cell catalog ID #291](https://dgrc.bio.indiana.edu/product/View?product=291) S2R+ with GFP::Lam. Ordering information: [DGRC cell catalog ID #292](https://dgrc.bio.indiana.edu/product/View?product=292) S2R+ with GFP::Spin. Ordering information: [DGRC cell catalog ID #293](https://dgrc.bio.indiana.edu/product/View?product=293) S2R+ with GFP::Sec23. Ordering information: [DGRC cell catalog ID #294](https://dgrc.bio.indiana.edu/product/View?product=294) S2R+ with GFP::Tom20. Ordering information: [DGRC cell catalog ID #302](https://dgrc.bio.indiana.edu/product/View?product=302) These cell lines were made using constructs designed and provided by Kanca and Bellen (Baylor College of Medicine). The cell lines were engineered, isolated, and validated at the DRSC. Validation testing included live-cell imaging, fixed-cell imaging (co-stained with an antibody, when possible), and molecular characterization of the insertion endpoints. ***S**ee the file attached below for a slide presentation with images of live and fixed cells***, as well as other relevant information about the approach and the resulting GFP-tagged cell lines. In addition, C-terminal GFP knock-in cell lines were generated as described in a BioRxiv preprint from [Bosch et al. (2019)](https://www.biorxiv.org/content/10.1101/639484v1) using an 'armless' donor approach. Act5c::GFP, Tub84B::GFP, His2Av::GFP, and Lamin::GFP fusion cell lines were made using this approach and are being shared with the DGRC for distribution to the community. **Knockout cell lines** S2R+-ZnT63C-KO, NHEJ-mediated knockout of *ZnT63C*. As described in [PMID: 29223976](https://www.ncbi.nlm.nih.gov/pubmed/29223976). Ordering information: [DGRC cell catalog #265](https://dgrc.bio.indiana.edu/product/View?product=265). S2R+-IA2-KO, NHEJ-mediated knockout of *ia2*. As described in [PMID: 29223976](https://www.ncbi.nlm.nih.gov/pubmed/29223976). Ordering information: [DGRC cell catalog #266](https://dgrc.bio.indiana.edu/product/View?product=266). S2R+-Apc-KO, two independent cell lines made with one method, [DGRC cell catalog #271](https://dgrc.bio.indiana.edu/product/View?product=271) and [DGRC cell catalog #272](https://dgrc.bio.indiana.edu/product/View?product=272) S2R+-Apc-KO, one additional cell line made with a different method, [DGRC cell catalog #270](https://dgrc.bio.indiana.edu/product/View?product=270) S2R+-gig-KO, [DGRC cell catalog #297](https://dgrc.bio.indiana.edu/product/View?product=297) S2R+-hairy-KO, [DGRC cell catalog #298](https://dgrc.bio.indiana.edu/product/View?product=298) S2R+-Moe-KO, [DGRC cell catalog #303](https://dgrc.bio.indiana.edu/product/View?product=303) S2R+-Pex19-KO, [DGRC cell catalog #306](https://dgrc.bio.indiana.edu/product/View?product=306) S2R+-Pten-KO, [DGRC cell catalog #307](https://dgrc.bio.indiana.edu/product/View?product=307) S2R+-Slik-KO, [DGRC cell catalog #308](https://dgrc.bio.indiana.edu/product/View?product=308) S2R+-Tnks-KO, three independent cell lines, [DGRC cell catalog #299](https://dgrc.bio.indiana.edu/product/View?product=299), [DGRC cell catalog #300](https://dgrc.bio.indiana.edu/product/View?product=300), and [DGRC cell catalog #301](https://dgrc.bio.indiana.edu/product/View?product=301) S2R+-Tnks-KO, one additional cell line made with a different method, [DGRC cell catalog #304](https://dgrc.bio.indiana.edu/product/View?product=304) S2R+-TSC1-KO, [DGRC cell catalog #305](https://dgrc.bio.indiana.edu/product/View?product=305) S2R+-Yki-KO, [DGRC cell catalog #309](https://dgrc.bio.indiana.edu/product/View?product=309) These cell lines have been sequence verified as containing only knockout alleles by PCR amplification of the target region followed by next-generation sequencing of the PCR amplicon, contig assembly, and comparison with the wild-type reference sequence (or, for KO alleles generated via knock-in, verified using PCR validation of the insertion allele). We wanted to make sure that the distribution copies of the cell lines are correct. To do this, for a subset of these cell lines, (1) the DGRC prepared genomic DNA from their distribution copies of the cell lines, (2) they shipped that gDNA to the DRSC/TRiP, and (3) we used that gDNA as template for PCR and NGS, and validated that all alleles are predicted to be gene knockout alleles. Information relevant to re-validation in your own lab is provided at the DGRC website. ***Did you request GFP-tagged or KO cells from the DGRC and use them in a study?*** If so, **please acknowledge both the cell line developers (DRSC) and the distribtors (DGRC)** by citing NIH Grant 5R24OD019847, which supported production of the resource at DRSC/TRiP, and the Drosophila Genome Resource Center, NIH grant 2P40OD010949, as well as relevant pulications. ***Slide presentation file -- GFP-tagged cell lines:*** ## Publications Download 12 citations download- [BibTeX](/bibcite/export?pager_style=no_pager&number_of_items=12&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114121&&&format=bibtex) - [EndNote X3 XML](/bibcite/export?pager_style=no_pager&number_of_items=12&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114121&&&format=endnote8) - [EndNote 7 XML](/bibcite/export?pager_style=no_pager&number_of_items=12&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114121&&&format=endnote7) - [Endnote tagged](/bibcite/export?pager_style=no_pager&number_of_items=12&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114121&&&format=tagged) - [Marc](/bibcite/export?pager_style=no_pager&number_of_items=12&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114121&&&format=marc) - [PubMedId](/bibcite/export?pager_style=no_pager&number_of_items=12&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114121&&&format=pubmed_id) - [RIS](/bibcite/export?pager_style=no_pager&number_of_items=12&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114121&&&format=ris) ### 2024 Enzo Mameli, George-Rafael Samantsidis, Raghuvir Viswanatha, Hyeogsun Kwon, David R Hall, Matthew Butnaru, Yanhui Hu, Stephanie E Mohr, Norbert Perrimon, and Ryan C Smith. 2024. “[A Genome-Wide CRISPR Screen in Mosquito Cells Identifies Essential Genes and Required Components of Clodronate Liposome Function.](/publication/genome-wide-crispr-screen-mosquito-cells-identifies-essential-genes-and-required)”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.24.614595 Enzo Mameli, George-Rafael Samantsidis, Raghuvir Viswanatha, Hyeogsun Kwon, David R Hall, Matthew Butnaru, Yanhui Hu, Stephanie E Mohr, Norbert Perrimon, and Ryan C Smith. 2024. “[A Genome-Wide CRISPR Screen in Mosquito Cells Identifies Essential Genes and Required Components of Clodronate Liposome Function.](/publication/genome-wide-crispr-screen-mosquito-cells-identifies-essential-genes-and-required)”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.24.614595 - add\_circle do\_not\_disturb\_on Abstract mosquitoes are the sole vector of human malaria, the most burdensome vector-borne disease worldwide. Strategies aimed at reducing mosquito populations and limiting their ability to transmit disease show the most promise for disease control. Therefore... Raghuvir Viswanatha, Samuel Entwisle, Claire Hu, Kelly Reap, Matthew Butnaru, Stephanie E Mohr, and Norbert Perrimon. 2024. “[Higher Resolution Pooled Genome-Wide CRISPR Knockout Screening in Drosophila Cells Using Integration and Anti-CRISPR (IntAC).](/publication/higher-resolution-pooled-genome-wide-crispr-knockout-screening-drosophila-cells-using)”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.19.613976 Raghuvir Viswanatha, Samuel Entwisle, Claire Hu, Kelly Reap, Matthew Butnaru, Stephanie E Mohr, and Norbert Perrimon. 2024. “[Higher Resolution Pooled Genome-Wide CRISPR Knockout Screening in Drosophila Cells Using Integration and Anti-CRISPR (IntAC).](/publication/higher-resolution-pooled-genome-wide-crispr-knockout-screening-drosophila-cells-using)”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.19.613976 - add\_circle do\_not\_disturb\_on Abstract CRISPR screens enable systematic, scalable genotype-to-phenotype mapping. We previously developed a pooled CRISPR screening method for and mosquito cell lines using plasmid transfection and site-specific integration to introduce single guide (sgRNA)... ### 2023 Baolong Xia, Raghuvir Viswanatha, Yanhui Hu, Stephanie Mohr, and Norbert Perrimon. 2023. “[Pooled Genome-Wide CRISPR Activation Screening for Rapamycin Resistance Genes in Cells](/publications/pooled-genome-wide-crispr-activation-screening-rapamycin-resistance-genes-cells)”. Elife, 12. doi:10.7554/eLife.85542 Baolong Xia, Raghuvir Viswanatha, Yanhui Hu, Stephanie Mohr, and Norbert Perrimon. 2023. “[Pooled Genome-Wide CRISPR Activation Screening for Rapamycin Resistance Genes in Cells](/publications/pooled-genome-wide-crispr-activation-screening-rapamycin-resistance-genes-cells)”. Elife, 12. doi:10.7554/eLife.85542 - add\_circle do\_not\_disturb\_on Abstract - [ picture\_as\_pdfelife-85542-v1.pdf](/sites/g/files/omnuum5366/files/fly/files/elife-85542-v1.pdf) Loss-of-function and gain-of-function genetic perturbations provide valuable insights into gene function. In cells, while genome-wide loss-of-function screens have been extensively used to reveal mechanisms of a variety of biological processes... - [ picture\_as\_pdfelife-85542-v1.pdf](/sites/g/files/omnuum5366/files/fly/files/elife-85542-v1.pdf) Agustin Rolandelli, Hanna Laukaitis-Yousey, Haikel Bogale, Nisha Singh, Sourabh Samaddar, Anya O’Neal, Camila Ferraz, Matthew Butnaru, Enzo Mameli, Baolong Xia, Tays Mendes, Rainer Butler, Liron Marnin, Francy Paz, Luisa Valencia, Vipin Rana, Ciaran Skerry, Utpal Pal, Stephanie Mohr, Norbert Perrimon, David Serre, and Joao Pedra. 2023. “[Tick Hemocytes Have Pleiotropic Roles in Microbial Infection and Arthropod Fitness](/publications/tick-hemocytes-have-pleiotropic-roles-microbial-infection-and-arthropod-fitness)”. BioRxiv, Pp. 2023.08.31.555785 Agustin Rolandelli, Hanna Laukaitis-Yousey, Haikel Bogale, Nisha Singh, Sourabh Samaddar, Anya O’Neal, Camila Ferraz, Matthew Butnaru, Enzo Mameli, Baolong Xia, Tays Mendes, Rainer Butler, Liron Marnin, Francy Paz, Luisa Valencia, Vipin Rana, Ciaran Skerry, Utpal Pal, Stephanie Mohr, Norbert Perrimon, David Serre, and Joao Pedra. 2023. “[Tick Hemocytes Have Pleiotropic Roles in Microbial Infection and Arthropod Fitness](/publications/tick-hemocytes-have-pleiotropic-roles-microbial-infection-and-arthropod-fitness)”. BioRxiv, Pp. 2023.08.31.555785 - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](http://biorxiv.org/content/early/2023/09/03/2023.08.31.555785.abstract) - [ picture\_as\_pdf2023.08.31.555785v1.full\_...](/sites/g/files/omnuum5366/files/fly/files/2023.08.31.555785v1.full_.pdf) Uncovering the complexity of systems in non-model organisms is critical for understanding arthropod immunology. Prior efforts have mostly focused on Dipteran insects, which only account for a subset of existing arthropod species in nature. Here, we... - [ descriptionPublisher's Version](http://biorxiv.org/content/early/2023/09/03/2023.08.31.555785.abstract) - [ picture\_as\_pdf2023.08.31.555785v1.full\_...](/sites/g/files/omnuum5366/files/fly/files/2023.08.31.555785v1.full_.pdf) Nisha Singh, Agustin Rolandelli, Anya O’Neal, Rainer Butler, Sourabh Samaddar, Hanna Laukaitis-Yousey, Matthew Butnaru, Stephanie Mohr, Norbert Perrimon, and Joao Pedra. 2023. “[Genetic Manipulation of an Ixodes Scapularis Cell Line](/publications/genetic-manipulation-ixodes-scapularis-cell-line)”. BioRxiv, Pp. 2023.09.08.556855 Nisha Singh, Agustin Rolandelli, Anya O’Neal, Rainer Butler, Sourabh Samaddar, Hanna Laukaitis-Yousey, Matthew Butnaru, Stephanie Mohr, Norbert Perrimon, and Joao Pedra. 2023. “[Genetic Manipulation of an Ixodes Scapularis Cell Line](/publications/genetic-manipulation-ixodes-scapularis-cell-line)”. BioRxiv, Pp. 2023.09.08.556855 - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](http://biorxiv.org/content/early/2023/09/10/2023.09.08.556855.abstract) - [ picture\_as\_pdf2023.09.08.556855v1.full\_...](/sites/g/files/omnuum5366/files/fly/files/2023.09.08.556855v1.full_.pdf) Although genetic manipulation is one of the hallmarks in model organisms, its applicability to non-model species has remained difficult due to our limited understanding of their fundamental biology. For instance, manipulation of a cell line originated... - [ descriptionPublisher's Version](http://biorxiv.org/content/early/2023/09/10/2023.09.08.556855.abstract) - [ picture\_as\_pdf2023.09.08.556855v1.full\_...](/sites/g/files/omnuum5366/files/fly/files/2023.09.08.556855v1.full_.pdf) ### 2022 Ying Xu, Raghuvir Viswanatha, Oleg Sitsel, Daniel Roderer, Haifang Zhao, Christopher Ashwood, Cecilia Voelcker, Songhai Tian, Stefan Raunser, Norbert Perrimon, and Min Dong. 2022. “[CRISPR Screens in Drosophila Cells Identify Vsg As a Tc Toxin Receptor](/publications/crispr-screens-drosophila-cells-identify-vsg-tc-toxin-receptor)”. Nature, 610, 7931, Pp. 349-55. doi:10.1038/s41586-022-05250-7 Ying Xu, Raghuvir Viswanatha, Oleg Sitsel, Daniel Roderer, Haifang Zhao, Christopher Ashwood, Cecilia Voelcker, Songhai Tian, Stefan Raunser, Norbert Perrimon, and Min Dong. 2022. “[CRISPR Screens in Drosophila Cells Identify Vsg As a Tc Toxin Receptor](/publications/crispr-screens-drosophila-cells-identify-vsg-tc-toxin-receptor)”. Nature, 610, 7931, Pp. 349-55. doi:10.1038/s41586-022-05250-7 - add\_circle do\_not\_disturb\_on Abstract Entomopathogenic nematodes are widely used as biopesticides1,2. Their insecticidal activity depends on symbiotic bacteria such as Photorhabdus luminescens, which produces toxin complex (Tc) toxins as major virulence factors3-6. No protein receptors are... Hans Dalton, Raghuvir Viswanatha, Roderick Brathwaite, Jae Sophia Zuno, Alexys Berman, Rebekah Rushforth, Stephanie Mohr, Norbert Perrimon, and Clement Chow. 2022. “[A Genome-Wide CRISPR Screen Identifies DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress](/publications/genome-wide-crispr-screen-identifies-dpm1-modifier-dpagt1-deficiency-and-er-stress)”. PLoS Genet, 18, 9, Pp. e1010430. doi:10.1371/journal.pgen.1010430 Hans Dalton, Raghuvir Viswanatha, Roderick Brathwaite, Jae Sophia Zuno, Alexys Berman, Rebekah Rushforth, Stephanie Mohr, Norbert Perrimon, and Clement Chow. 2022. “[A Genome-Wide CRISPR Screen Identifies DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress](/publications/genome-wide-crispr-screen-identifies-dpm1-modifier-dpagt1-deficiency-and-er-stress)”. PLoS Genet, 18, 9, Pp. e1010430. doi:10.1371/journal.pgen.1010430 - add\_circle do\_not\_disturb\_on Abstract Partial loss-of-function mutations in glycosylation pathways underlie a set of rare diseases called Congenital Disorders of Glycosylation (CDGs). In particular, DPAGT1-CDG is caused by mutations in the gene encoding the first step in N-glycosylation... Jonathan Zirin, Justin Bosch, Raghuvir Viswanatha, Stephanie Mohr, and Norbert Perrimon. 2022. “[State-of-the-Art CRISPR for in Vivo and Cell-Based Studies in Drosophila](/publications/state-art-crispr-vivo-and-cell-based-studies-drosophila)”. Trends Genet, 38, 5, Pp. 437-53. doi:10.1016/j.tig.2021.11.006 Jonathan Zirin, Justin Bosch, Raghuvir Viswanatha, Stephanie Mohr, and Norbert Perrimon. 2022. “[State-of-the-Art CRISPR for in Vivo and Cell-Based Studies in Drosophila](/publications/state-art-crispr-vivo-and-cell-based-studies-drosophila)”. Trends Genet, 38, 5, Pp. 437-53. doi:10.1016/j.tig.2021.11.006 - add\_circle do\_not\_disturb\_on Abstract For more than 100 years, the fruit fly, Drosophila melanogaster, has served as a powerful model organism for biological and biomedical research due to its many genetic and physiological similarities to humans and the availability of sophisticated... ### 2021 Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya Colpitts, Stephanie Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “[Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos](/publications/bioinformatic-and-cell-based-tools-pooled-crispr-knockout-screening-mosquitos-0)”. Nat Commun, 12, 1, Pp. 6825. doi:10.1038/s41467-021-27129-3 Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya Colpitts, Stephanie Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “[Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos](/publications/bioinformatic-and-cell-based-tools-pooled-crispr-knockout-screening-mosquitos-0)”. Nat Commun, 12, 1, Pp. 6825. doi:10.1038/s41467-021-27129-3 - add\_circle do\_not\_disturb\_on Abstract - [ picture\_as\_pdfs41467-021-27129-31.pdf](/sites/g/files/omnuum5366/files/fly/files/s41467-021-27129-31.pdf) Mosquito-borne diseases present a worldwide public health burden. Current efforts to understand and counteract them have been aided by the use of cultured mosquito cells. Moreover, application in mammalian cells of forward genetic approaches such as... - [ picture\_as\_pdfs41467-021-27129-31.pdf](/sites/g/files/omnuum5366/files/fly/files/s41467-021-27129-31.pdf) J. A. Bosch, G. Birchak, and N. Perrimon. 2021. “[Precise Genome Engineering in Drosophila Using Prime Editing](/publications/precise-genome-engineering-drosophila-using-prime-editing)”. Proc Natl Acad Sci U S A, 118 J. A. Bosch, G. Birchak, and N. Perrimon. 2021. “[Precise Genome Engineering in Drosophila Using Prime Editing](/publications/precise-genome-engineering-drosophila-using-prime-editing)”. Proc Natl Acad Sci U S A, 118 - add\_circle do\_not\_disturb\_on Abstract Precise genome editing is a valuable tool to study gene function in model organisms. Prime editing, a precise editing system developed in mammalian cells, does not require double-strand breaks or donor DNA and has low off-target effects. Here, we applied... Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya M. Colpitts, Stephanie E. Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “[Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos \[NOTE: A Modified Final Version Was Published in Nat Comm and Is Now Available.\]](/publications/bioinformatic-and-cell-based-tools-pooled-crispr-knockout-screening-mosquitos)”. BioRxiv. doi:10.1101/2021.03.29.437496 Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya M. Colpitts, Stephanie E. Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “[Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos \[NOTE: A Modified Final Version Was Published in Nat Comm and Is Now Available.\]](/publications/bioinformatic-and-cell-based-tools-pooled-crispr-knockout-screening-mosquitos)”. BioRxiv. doi:10.1101/2021.03.29.437496 - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](https://www.biorxiv.org/content/early/2021/03/30/2021.03.29.437496) - [ picture\_as\_pdf2021.03.29.437496v2.full\_...](/sites/g/files/omnuum5366/files/fly/files/2021.03.29.437496v2.full_.pdf) Mosquito-borne diseases present a worldwide public health burden. Genome-scale screening tools that could inform our understanding of mosquitos and their control are lacking. Here, we adapt a recombination-mediated cassette exchange system for delivery of... - [ descriptionPublisher's Version](https://www.biorxiv.org/content/early/2021/03/30/2021.03.29.437496) - [ picture\_as\_pdf2021.03.29.437496v2.full\_...](/sites/g/files/omnuum5366/files/fly/files/2021.03.29.437496v2.full_.pdf) Hans M. Dalton, Raghuvir Viswanatha, Ricky Brathwaite Jr., Jae Sophia Zuno, Stephanie Mohr, Norbert Perrimon, and Clement Y. Chow. 2021. “[A Genome-Wide CRISPR Screen Identifies the Glycosylation Enzyme DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress](/publications/genome-wide-crispr-screen-identifies-glycosylation-enzyme-dpm1-modifier-dpagt1)”. BioRxiv Hans M. Dalton, Raghuvir Viswanatha, Ricky Brathwaite Jr., Jae Sophia Zuno, Stephanie Mohr, Norbert Perrimon, and Clement Y. Chow. 2021. “[A Genome-Wide CRISPR Screen Identifies the Glycosylation Enzyme DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress](/publications/genome-wide-crispr-screen-identifies-glycosylation-enzyme-dpm1-modifier-dpagt1)”. BioRxiv - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](https://www.biorxiv.org/content/10.1101/2021.12.03.471178v1) - [ picture\_as\_pdf2021.12.03.471178v1.full\_...](/sites/g/files/omnuum5366/files/fly/files/2021.12.03.471178v1.full_.pdf) Partial loss-of-function mutations in glycosylation pathways underlie a set of rare diseases called Congenital Disorders of Glycosylation (CDGs). In particular, DPAGT1 CDG is caused by mutations in the gene encoding the first step in N-glycosylation... - [ descriptionPublisher's Version](https://www.biorxiv.org/content/10.1101/2021.12.03.471178v1) - [ picture\_as\_pdf2021.12.03.471178v1.full\_...](/sites/g/files/omnuum5366/files/fly/files/2021.12.03.471178v1.full_.pdf) [ More arrow\_circle\_right ](/publications/technology/fly-cell-based-crispr) --- Attachments- [ picture\_as\_pdf smohr\_drsc\_gfp-tag\_websharecopy.pdf ](/sites/g/files/omnuum5366/files/fly/files/smohr_drsc_gfp-tag_websharecopy.pdf) ---