#  in vivo CRISPR fly stocks 

 



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 The TRiP-CRISPR project aims to build genome scale collections of transgenic sgRNA fly lines, providing powerful, versatile and transformative tools for the fly community. For a detailed description of the the TRiP in vivo CRISPR approach click [here](/vivo-crispr-0).

Sort    TRiP-CRISPR fly stock collections

   **Function**

   **sgRNA Vectors**

   **Cross to**

   **Notes**

       [TRiP-OE](/trip-overexpression-stocks)

 

  [VPR](/trip-overexpression-stocks)

 

  Gene Activation

 

  [pCFD4](/sgrna-vectors)

 

  Gal4+dCas9-VPR

 

  See [TRiP-CRISPR Toolbox](/trip-crispr-toolbox-fly-stocks)

 

    [flySAM1.0](/trip-overexpression-stocks)

 

   Gene Activation

 

  [U6B-sgRNA2.0](/sgrna-vectors)

 

  Gal4+flySAM

 

  See [TRiP-CRISPR Toolbox](/trip-crispr-toolbox-fly-stocks)

 

    [flySAM2.0](/trip-overexpression-stocks)

 

  Gene Activation

 

  [flySAM2.0](/sgrna-vectors)

 

  Gal4

 

  flySAM2.0 lines contain both sgRNA and dCas9 (flySAM)

 

    [TRiP-KO](/trip-knockout)

 

   Gene Cutting

 

  [pCFD3](/sgrna-vectors), [pCFD4](/sgrna-vectors)

 

  Gal4+Cas9

 

  See [TRiP-CRISPR Toolbox](/trip-crispr-toolbox-fly-stocks)

 

 



##  **[Search](http://www.flyrnai.org/tools/grna_tracker/web/)** the DRSC/TRiP sgRNA Stock Database for TRiP-OE and TRiP-KO stocks

##  [**Nominate genes for production** ](http://www.flyrnai.org/tools/grna_tracker/web/nominate)for TRiP-OE and TRiP-KO stocks

##  **[Download](http://www.flyrnai.org/tools/grna_tracker/web/grna_tracker_finished_stocks.csv)** a .XLS file of currently available TRiP-OE and TRiP-KO stocks

- [TRiP-CRISPR Overexpression (TRiP-OE)](/trip-overexpression-stocks) - for gene activation in somatic and germline cells
- [TRiP-CRISPR Knockout (TRiP-KO)](/trip-knockout) - for mutagenesis in somatic and germline cells
- [TRiP-CRISPR toolbox](/trip-crispr-toolbox-fly-stocks) - for stage- and tissue-specific Cas9 expression
- [Prime editing](/prime-editing-reagents) - for precise genome editing in somatic and germline cells

###  Community Access to TRiP-CRISPR Stocks

 The TRiP-CRISPR project leverages the existing transgenic RNAi platform to produce the stocks, making them available at the [Bloomington Drosophila Stock Center](https://bdsc.indiana.edu/index.html) (BDSC), and curating information on the quality of reagents via the DRSC/TRiP website. As we build the new CRISPR collection, we will encourage and receive gene target nominations from the community.

- All completed stocks are annotated on the DRSC/TRiP website and on FlyBase, and transferred as soon as possible to the BDSC for distribution to the community
- If a TRiP-CRISPR stock has not yet been deposited at the BDSC, please contact the [TRiP facility at HMS](mailto:///TRiP@flyrnai.org) and we will send it to you
- If use of any of the TRiP-CRISPR stocks results in data that is included in a manuscript for publication, the TRiP requests that a version of the following statement be included in the Acknowledgements section: "We thank the TRiP at Harvard Medical School (NIH/NIGMS R01-GM084947) for providing transgenic sgRNA fly stocks used in this study"

###  Nominating genes for TRiP-CRISPR production

- Nominate genes for TRiP-OE and TRiP-KO production via the [DRSC/TRiP sgRNA Stock Tracking System](http://www.flyrnai.org/tools/grna_tracker/)
- We will meet the needs of the Drosophila community for in vivo phenotypic analyses
- We will meet the needs of screeners at the Drosophila RNAi Screening Center (DRSC) for in vivo follow-up studies subsequent to cell-based screens