# Cell CRISPR screen reagents ## expand\_more Three sgRNA libraries for CRISPR knockout screening in *Drosophila* cells that together cover the full *Drosophila* genome [are available from Addgene](https://www.addgene.org/pooled-library/perrimon-drosophila-crispr-knockout/). A Cas9 cell line useful for screening is [available at the DGRC](https://dgrc.bio.indiana.edu/product/View?product=268). We also published a protocol manuscript in *Current Protocols in Molecular Biology* describing the pooled screen process in detail (Viswanatha et al. 2019; please see below). We are here to help! Please reach out to DRSC Director S. Mohr for more information about pooled-format screening in *Drosophila* cultured cells. We are developing new assays, libraries, and cell lines, and we welcome inquiries about these new technologies. ![CRISPR knockout screening in Drosophila cells](/sites/g/files/omnuum5366/files/fly/files/crispr_pooled_screening_2022-04_DRSC-BTRR.png) ## Publications Download 12 citations download- [BibTeX](/bibcite/export?pager_style=no_pager&number_of_items=12&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114121&&&format=bibtex) - [EndNote X3 XML](/bibcite/export?pager_style=no_pager&number_of_items=12&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114121&&&format=endnote8) - [EndNote 7 XML](/bibcite/export?pager_style=no_pager&number_of_items=12&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114121&&&format=endnote7) - [Endnote tagged](/bibcite/export?pager_style=no_pager&number_of_items=12&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114121&&&format=tagged) - [Marc](/bibcite/export?pager_style=no_pager&number_of_items=12&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114121&&&format=marc) - [PubMedId](/bibcite/export?pager_style=no_pager&number_of_items=12&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114121&&&format=pubmed_id) - [RIS](/bibcite/export?pager_style=no_pager&number_of_items=12&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114121&&&format=ris) ### 2024 Enzo Mameli, George-Rafael Samantsidis, Raghuvir Viswanatha, Hyeogsun Kwon, David R Hall, Matthew Butnaru, Yanhui Hu, Stephanie E Mohr, Norbert Perrimon, and Ryan C Smith. 2024. “[A Genome-Wide CRISPR Screen in Mosquito Cells Identifies Essential Genes and Required Components of Clodronate Liposome Function.](/publication/genome-wide-crispr-screen-mosquito-cells-identifies-essential-genes-and-required)”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.24.614595 Enzo Mameli, George-Rafael Samantsidis, Raghuvir Viswanatha, Hyeogsun Kwon, David R Hall, Matthew Butnaru, Yanhui Hu, Stephanie E Mohr, Norbert Perrimon, and Ryan C Smith. 2024. “[A Genome-Wide CRISPR Screen in Mosquito Cells Identifies Essential Genes and Required Components of Clodronate Liposome Function.](/publication/genome-wide-crispr-screen-mosquito-cells-identifies-essential-genes-and-required)”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.24.614595 - add\_circle do\_not\_disturb\_on Abstract mosquitoes are the sole vector of human malaria, the most burdensome vector-borne disease worldwide. Strategies aimed at reducing mosquito populations and limiting their ability to transmit disease show the most promise for disease control. Therefore... Raghuvir Viswanatha, Samuel Entwisle, Claire Hu, Kelly Reap, Matthew Butnaru, Stephanie E Mohr, and Norbert Perrimon. 2024. “[Higher Resolution Pooled Genome-Wide CRISPR Knockout Screening in Drosophila Cells Using Integration and Anti-CRISPR (IntAC).](/publication/higher-resolution-pooled-genome-wide-crispr-knockout-screening-drosophila-cells-using)”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.19.613976 Raghuvir Viswanatha, Samuel Entwisle, Claire Hu, Kelly Reap, Matthew Butnaru, Stephanie E Mohr, and Norbert Perrimon. 2024. “[Higher Resolution Pooled Genome-Wide CRISPR Knockout Screening in Drosophila Cells Using Integration and Anti-CRISPR (IntAC).](/publication/higher-resolution-pooled-genome-wide-crispr-knockout-screening-drosophila-cells-using)”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.19.613976 - add\_circle do\_not\_disturb\_on Abstract CRISPR screens enable systematic, scalable genotype-to-phenotype mapping. We previously developed a pooled CRISPR screening method for and mosquito cell lines using plasmid transfection and site-specific integration to introduce single guide (sgRNA)... ### 2023 Baolong Xia, Raghuvir Viswanatha, Yanhui Hu, Stephanie Mohr, and Norbert Perrimon. 2023. “[Pooled Genome-Wide CRISPR Activation Screening for Rapamycin Resistance Genes in Cells](/publications/pooled-genome-wide-crispr-activation-screening-rapamycin-resistance-genes-cells)”. Elife, 12. doi:10.7554/eLife.85542 Baolong Xia, Raghuvir Viswanatha, Yanhui Hu, Stephanie Mohr, and Norbert Perrimon. 2023. “[Pooled Genome-Wide CRISPR Activation Screening for Rapamycin Resistance Genes in Cells](/publications/pooled-genome-wide-crispr-activation-screening-rapamycin-resistance-genes-cells)”. Elife, 12. doi:10.7554/eLife.85542 - add\_circle do\_not\_disturb\_on Abstract - [ picture\_as\_pdfelife-85542-v1.pdf](/sites/g/files/omnuum5366/files/fly/files/elife-85542-v1.pdf) Loss-of-function and gain-of-function genetic perturbations provide valuable insights into gene function. In cells, while genome-wide loss-of-function screens have been extensively used to reveal mechanisms of a variety of biological processes... - [ picture\_as\_pdfelife-85542-v1.pdf](/sites/g/files/omnuum5366/files/fly/files/elife-85542-v1.pdf) Agustin Rolandelli, Hanna Laukaitis-Yousey, Haikel Bogale, Nisha Singh, Sourabh Samaddar, Anya O’Neal, Camila Ferraz, Matthew Butnaru, Enzo Mameli, Baolong Xia, Tays Mendes, Rainer Butler, Liron Marnin, Francy Paz, Luisa Valencia, Vipin Rana, Ciaran Skerry, Utpal Pal, Stephanie Mohr, Norbert Perrimon, David Serre, and Joao Pedra. 2023. “[Tick Hemocytes Have Pleiotropic Roles in Microbial Infection and Arthropod Fitness](/publications/tick-hemocytes-have-pleiotropic-roles-microbial-infection-and-arthropod-fitness)”. BioRxiv, Pp. 2023.08.31.555785 Agustin Rolandelli, Hanna Laukaitis-Yousey, Haikel Bogale, Nisha Singh, Sourabh Samaddar, Anya O’Neal, Camila Ferraz, Matthew Butnaru, Enzo Mameli, Baolong Xia, Tays Mendes, Rainer Butler, Liron Marnin, Francy Paz, Luisa Valencia, Vipin Rana, Ciaran Skerry, Utpal Pal, Stephanie Mohr, Norbert Perrimon, David Serre, and Joao Pedra. 2023. “[Tick Hemocytes Have Pleiotropic Roles in Microbial Infection and Arthropod Fitness](/publications/tick-hemocytes-have-pleiotropic-roles-microbial-infection-and-arthropod-fitness)”. BioRxiv, Pp. 2023.08.31.555785 - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](http://biorxiv.org/content/early/2023/09/03/2023.08.31.555785.abstract) - [ picture\_as\_pdf2023.08.31.555785v1.full\_...](/sites/g/files/omnuum5366/files/fly/files/2023.08.31.555785v1.full_.pdf) Uncovering the complexity of systems in non-model organisms is critical for understanding arthropod immunology. Prior efforts have mostly focused on Dipteran insects, which only account for a subset of existing arthropod species in nature. Here, we... - [ descriptionPublisher's Version](http://biorxiv.org/content/early/2023/09/03/2023.08.31.555785.abstract) - [ picture\_as\_pdf2023.08.31.555785v1.full\_...](/sites/g/files/omnuum5366/files/fly/files/2023.08.31.555785v1.full_.pdf) Nisha Singh, Agustin Rolandelli, Anya O’Neal, Rainer Butler, Sourabh Samaddar, Hanna Laukaitis-Yousey, Matthew Butnaru, Stephanie Mohr, Norbert Perrimon, and Joao Pedra. 2023. “[Genetic Manipulation of an Ixodes Scapularis Cell Line](/publications/genetic-manipulation-ixodes-scapularis-cell-line)”. BioRxiv, Pp. 2023.09.08.556855 Nisha Singh, Agustin Rolandelli, Anya O’Neal, Rainer Butler, Sourabh Samaddar, Hanna Laukaitis-Yousey, Matthew Butnaru, Stephanie Mohr, Norbert Perrimon, and Joao Pedra. 2023. “[Genetic Manipulation of an Ixodes Scapularis Cell Line](/publications/genetic-manipulation-ixodes-scapularis-cell-line)”. BioRxiv, Pp. 2023.09.08.556855 - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](http://biorxiv.org/content/early/2023/09/10/2023.09.08.556855.abstract) - [ picture\_as\_pdf2023.09.08.556855v1.full\_...](/sites/g/files/omnuum5366/files/fly/files/2023.09.08.556855v1.full_.pdf) Although genetic manipulation is one of the hallmarks in model organisms, its applicability to non-model species has remained difficult due to our limited understanding of their fundamental biology. For instance, manipulation of a cell line originated... - [ descriptionPublisher's Version](http://biorxiv.org/content/early/2023/09/10/2023.09.08.556855.abstract) - [ picture\_as\_pdf2023.09.08.556855v1.full\_...](/sites/g/files/omnuum5366/files/fly/files/2023.09.08.556855v1.full_.pdf) ### 2022 Ying Xu, Raghuvir Viswanatha, Oleg Sitsel, Daniel Roderer, Haifang Zhao, Christopher Ashwood, Cecilia Voelcker, Songhai Tian, Stefan Raunser, Norbert Perrimon, and Min Dong. 2022. “[CRISPR Screens in Drosophila Cells Identify Vsg As a Tc Toxin Receptor](/publications/crispr-screens-drosophila-cells-identify-vsg-tc-toxin-receptor)”. Nature, 610, 7931, Pp. 349-55. doi:10.1038/s41586-022-05250-7 Ying Xu, Raghuvir Viswanatha, Oleg Sitsel, Daniel Roderer, Haifang Zhao, Christopher Ashwood, Cecilia Voelcker, Songhai Tian, Stefan Raunser, Norbert Perrimon, and Min Dong. 2022. “[CRISPR Screens in Drosophila Cells Identify Vsg As a Tc Toxin Receptor](/publications/crispr-screens-drosophila-cells-identify-vsg-tc-toxin-receptor)”. Nature, 610, 7931, Pp. 349-55. doi:10.1038/s41586-022-05250-7 - add\_circle do\_not\_disturb\_on Abstract Entomopathogenic nematodes are widely used as biopesticides1,2. Their insecticidal activity depends on symbiotic bacteria such as Photorhabdus luminescens, which produces toxin complex (Tc) toxins as major virulence factors3-6. No protein receptors are... Hans Dalton, Raghuvir Viswanatha, Roderick Brathwaite, Jae Sophia Zuno, Alexys Berman, Rebekah Rushforth, Stephanie Mohr, Norbert Perrimon, and Clement Chow. 2022. “[A Genome-Wide CRISPR Screen Identifies DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress](/publications/genome-wide-crispr-screen-identifies-dpm1-modifier-dpagt1-deficiency-and-er-stress)”. PLoS Genet, 18, 9, Pp. e1010430. doi:10.1371/journal.pgen.1010430 Hans Dalton, Raghuvir Viswanatha, Roderick Brathwaite, Jae Sophia Zuno, Alexys Berman, Rebekah Rushforth, Stephanie Mohr, Norbert Perrimon, and Clement Chow. 2022. “[A Genome-Wide CRISPR Screen Identifies DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress](/publications/genome-wide-crispr-screen-identifies-dpm1-modifier-dpagt1-deficiency-and-er-stress)”. PLoS Genet, 18, 9, Pp. e1010430. doi:10.1371/journal.pgen.1010430 - add\_circle do\_not\_disturb\_on Abstract Partial loss-of-function mutations in glycosylation pathways underlie a set of rare diseases called Congenital Disorders of Glycosylation (CDGs). In particular, DPAGT1-CDG is caused by mutations in the gene encoding the first step in N-glycosylation... Jonathan Zirin, Justin Bosch, Raghuvir Viswanatha, Stephanie Mohr, and Norbert Perrimon. 2022. “[State-of-the-Art CRISPR for in Vivo and Cell-Based Studies in Drosophila](/publications/state-art-crispr-vivo-and-cell-based-studies-drosophila)”. Trends Genet, 38, 5, Pp. 437-53. doi:10.1016/j.tig.2021.11.006 Jonathan Zirin, Justin Bosch, Raghuvir Viswanatha, Stephanie Mohr, and Norbert Perrimon. 2022. “[State-of-the-Art CRISPR for in Vivo and Cell-Based Studies in Drosophila](/publications/state-art-crispr-vivo-and-cell-based-studies-drosophila)”. Trends Genet, 38, 5, Pp. 437-53. doi:10.1016/j.tig.2021.11.006 - add\_circle do\_not\_disturb\_on Abstract For more than 100 years, the fruit fly, Drosophila melanogaster, has served as a powerful model organism for biological and biomedical research due to its many genetic and physiological similarities to humans and the availability of sophisticated... ### 2021 Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya Colpitts, Stephanie Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “[Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos](/publications/bioinformatic-and-cell-based-tools-pooled-crispr-knockout-screening-mosquitos-0)”. Nat Commun, 12, 1, Pp. 6825. doi:10.1038/s41467-021-27129-3 Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya Colpitts, Stephanie Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “[Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos](/publications/bioinformatic-and-cell-based-tools-pooled-crispr-knockout-screening-mosquitos-0)”. Nat Commun, 12, 1, Pp. 6825. doi:10.1038/s41467-021-27129-3 - add\_circle do\_not\_disturb\_on Abstract - [ picture\_as\_pdfs41467-021-27129-31.pdf](/sites/g/files/omnuum5366/files/fly/files/s41467-021-27129-31.pdf) Mosquito-borne diseases present a worldwide public health burden. Current efforts to understand and counteract them have been aided by the use of cultured mosquito cells. Moreover, application in mammalian cells of forward genetic approaches such as... - [ picture\_as\_pdfs41467-021-27129-31.pdf](/sites/g/files/omnuum5366/files/fly/files/s41467-021-27129-31.pdf) J. A. Bosch, G. Birchak, and N. Perrimon. 2021. “[Precise Genome Engineering in Drosophila Using Prime Editing](/publications/precise-genome-engineering-drosophila-using-prime-editing)”. Proc Natl Acad Sci U S A, 118 J. A. Bosch, G. Birchak, and N. Perrimon. 2021. “[Precise Genome Engineering in Drosophila Using Prime Editing](/publications/precise-genome-engineering-drosophila-using-prime-editing)”. Proc Natl Acad Sci U S A, 118 - add\_circle do\_not\_disturb\_on Abstract Precise genome editing is a valuable tool to study gene function in model organisms. Prime editing, a precise editing system developed in mammalian cells, does not require double-strand breaks or donor DNA and has low off-target effects. Here, we applied... Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya M. Colpitts, Stephanie E. Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “[Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos \[NOTE: A Modified Final Version Was Published in Nat Comm and Is Now Available.\]](/publications/bioinformatic-and-cell-based-tools-pooled-crispr-knockout-screening-mosquitos)”. BioRxiv. doi:10.1101/2021.03.29.437496 Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya M. Colpitts, Stephanie E. Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “[Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos \[NOTE: A Modified Final Version Was Published in Nat Comm and Is Now Available.\]](/publications/bioinformatic-and-cell-based-tools-pooled-crispr-knockout-screening-mosquitos)”. BioRxiv. doi:10.1101/2021.03.29.437496 - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](https://www.biorxiv.org/content/early/2021/03/30/2021.03.29.437496) - [ picture\_as\_pdf2021.03.29.437496v2.full\_...](/sites/g/files/omnuum5366/files/fly/files/2021.03.29.437496v2.full_.pdf) Mosquito-borne diseases present a worldwide public health burden. Genome-scale screening tools that could inform our understanding of mosquitos and their control are lacking. Here, we adapt a recombination-mediated cassette exchange system for delivery of... - [ descriptionPublisher's Version](https://www.biorxiv.org/content/early/2021/03/30/2021.03.29.437496) - [ picture\_as\_pdf2021.03.29.437496v2.full\_...](/sites/g/files/omnuum5366/files/fly/files/2021.03.29.437496v2.full_.pdf) Hans M. Dalton, Raghuvir Viswanatha, Ricky Brathwaite Jr., Jae Sophia Zuno, Stephanie Mohr, Norbert Perrimon, and Clement Y. Chow. 2021. “[A Genome-Wide CRISPR Screen Identifies the Glycosylation Enzyme DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress](/publications/genome-wide-crispr-screen-identifies-glycosylation-enzyme-dpm1-modifier-dpagt1)”. BioRxiv Hans M. Dalton, Raghuvir Viswanatha, Ricky Brathwaite Jr., Jae Sophia Zuno, Stephanie Mohr, Norbert Perrimon, and Clement Y. Chow. 2021. “[A Genome-Wide CRISPR Screen Identifies the Glycosylation Enzyme DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress](/publications/genome-wide-crispr-screen-identifies-glycosylation-enzyme-dpm1-modifier-dpagt1)”. BioRxiv - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](https://www.biorxiv.org/content/10.1101/2021.12.03.471178v1) - [ picture\_as\_pdf2021.12.03.471178v1.full\_...](/sites/g/files/omnuum5366/files/fly/files/2021.12.03.471178v1.full_.pdf) Partial loss-of-function mutations in glycosylation pathways underlie a set of rare diseases called Congenital Disorders of Glycosylation (CDGs). In particular, DPAGT1 CDG is caused by mutations in the gene encoding the first step in N-glycosylation... - [ descriptionPublisher's Version](https://www.biorxiv.org/content/10.1101/2021.12.03.471178v1) - [ picture\_as\_pdf2021.12.03.471178v1.full\_...](/sites/g/files/omnuum5366/files/fly/files/2021.12.03.471178v1.full_.pdf) [ More arrow\_circle\_right ](/publications/technology/fly-cell-based-crispr)