DRSC/TRiP and DRSC-BTRR Office Hours

September 13, 2021
Graphical image of tissue culture, fly pushing, and computer, and the team of people who work with them

New this fall: Online office hours!

Do you have questions about modifying Drosophila cell lines with CRISPR or performing large-scale cell screens? Questions about in vivo RNAi with TRiP fly stocks or CRISPR knockout or activation with our sgRNA fly stocks? Questions about our new protocols and resources for CRISPR mosquito cell lines? Pop into our Zoom office hours to say hello and get our expert input! Registration is required (see below).

DRSC/TRiP & DRSC-BTRR Office Hours Schedule:

Mon. Sept. 27, 2021, 12 pm EST, Drosophila cell technologies
[Missed it? View a summary info file
here.]

·      Drosophila cell culture and media
·      Drosophila cell transfection by lipofection or electroporation
·      Drosophila cell knockout, knock-in, and RNAi
·      Drosophila cell CRISPR knockout screening
·      Drosophila cell CRISPR activation screening
 
Mon. Oct. 4, 2021, 12 pm EST, Drosophila in vivo RNAi technologies
[Missed it? View a summary info file
here.]
·      In vivo RNAi knockdown using TRiP fly stocks
·      Controls and caveats to in vivo RNAi
·      RSVP Plus online resource for lookup of TRiP RNAi stock info
·      TRiP plasmid vectors
 
Mon. Oct. 18, 2021, 12 pm EST, Drosophila in vivo CRISPR technologies
[Missed it? View a summary info file
here.]
·      In vivo CRISPR knockout using TRiP fly stocks
·      In vivo CRISPR activation using TRiP fly stocks
·      RSVP Plus online resource for lookup of TRiP CRISPR stock info
·      In vivo prime editing
 
Mon. Nov. 1, 2021, 12 pm EST, Drosophila bioinformatics resources
[Missed it? See our user-centric NAR database issue paper here and 'tools overview' page here.]
·      Using DRSC online tools for building ‘gene lists’
·      Using DRSC online tools for designing and identifying reagents
·      Using DRSC online tools for data analysis and integration
·      Using DRSC online tools for data mining
 
Mon. Nov. 8, 2021, 12 pm EST, Mosquito CRISPR cell technologies
·      How we handle mosquito cell culture and media at the DRSC
·      Mosquito cell transfection by lipofection or electroporation
·      Modified mosquito cells for low- or high-throughput CRISPR modification
·      Mosquito cell CRISPR knockout screening
 
Registration:

https://forms.office.com/r/qqwHpcjxrS