For target gene mutagenesis
- Generate indels (small insertion or deletion mutations) in the soma by crossing to tissue-specific Gal4>Cas9 flies
- Generate indels in the germline by crossing to germline-specific-Cas9 flies
- For a detailed description of the TRiP-KO gene mutagenesis approach click here
Access TRiP-KO stocks
- Order TRiP-KO transgenic stocks at the Bloomington Drosophila Stock Center (BDSC)
- If a TRiP-CRISPR stock has not yet been deposited at the BDSC, please contact the TRiP facility at HMS and we will send it to you
- Download a .XLS file of currently available TRiP-CRISPR stocks
Search TRiP-KO stocks
- Search the DRSC/TRiP sgRNA Stock Tracking System for TRiP-KO stocks and production status, and nominate genes for production
About TRiP-KO stocks
- A single gene is targeted by expression of a single sgRNA from a U6 promoter
- Stocks are made in the pCFD3 vector, developed by Fillip Port and colleagues (http://www.crisprflydesign.org/plasmids)
- Crossing TRiP KO stocks to a Gal4 line expressing Cas9 induces cleavage of the target gene and mutagenesis via Non-Homologous End Joining (NHEJ) repair
- To learn more about the TRiP KO vectors, sgRNA design, and CRISPR mutagenesis please visit the in vivo CRISPR protocols page
- For the benefit of the research community, production of TRiP-KO stocks is coordinated with similar efforts at the German Cancer Research Center (http://www.crisprflydesign.org/) and The National Institute of Genetics, Japan (https://shigen.nig.ac.jp/fly/nigfly/cas9/)