@article {896531,
	title = {An Integrative Analysis of the InR/PI3K/Akt Network Identifies the Dynamic Response to Insulin Signaling},
	journal = {Cell Reports},
	volume = {16},
	year = {2016},
	month = {2016},
	pages = {3062-3074},
	abstract = {<p>Insulin regulates an essential conserved signaling pathway affecting growth, proliferation, and meta- bolism. To expand our understanding of the insulin pathway, we combine biochemical, genetic, and computational approaches to build a comprehensive Drosophila InR/PI3K/Akt network. First, we map the dynamic protein-protein interaction network sur- rounding the insulin core pathway using bait-prey interactions connecting 566 proteins. Combining RNAi screening and phospho-specific antibodies, we find that 47\% of interacting proteins affect pathway activity, and, using quantitative phospho- proteomics, we demonstrate that $10\% of interact- ing proteins are regulated by insulin stimulation at the level of phosphorylation. Next, we integrate these orthogonal datasets to characterize the structure and dynamics of the insulin network at the level of protein complexes and validate our method by iden- tifying regulatory roles for the Protein Phosphatase 2A (PP2A) and Reptin-Pontin chromatin-remodeling complexes as negative and positive regulators of ribosome biogenesis, respectively. Altogether, our study represents a comprehensive resource for the study of the evolutionary conserved insulin network.\&nbsp;</p>},
	author = {Vinayagam, Arunachalam and Kulkarni, Meghana M and Sopko, Richelle and Sun, Xiaoyun and Hu, Yanhui and Nand, Ankita and Villalta, Christians and Moghimi, Ahmadali and Yang, Xuemei and Mohr, Stephanie E and Hong, Pengyu and Asara, John M and Perrimon, Norbert}
}
