Fluorescence/Luminescence Plate Readers

Luminometers and fluorimeters allow for readout of luminescence (luciferase readouts) or fluorescence from an entire well of a micro-well plate such as a 96- or 384-well plate.

We have the following plate-reader instruments available for on-site screens and other projects

  • Molecular Devices Spectramax Paradigm for luminescence, fluorescence, and spectrophotometry
  • LiCor Aerius for far-red fluorescence including 'in cell Western' approaches

These instruments support

  • total luminescence (luciferase readouts)
  • total fluorescence in standard and tunable channels
  • spectrophotometry (e.g. for total protein assays)

The input material is typically cultured cells but can be crushed flies as well, such as for luciferase-based high-throughput in vivo readouts.

Additional plate readers are available through the HMS ICCB-Longwood Screening Facility.

Molecular Devices Spectramax Paradigm plate reader with plate stacker at the DRSC-FGR