UP-TORR Documentation

URL

http://www.flyrnai.org/up-torr/

Video Tutorial

Input Form

UP-TORR input form

UP-TORR search options

Note: Maximum rows of search input:500. For a report of all reagents, use the
"All-Reagent On-Target" and "All-Reagent Off-Target" download options on the Fly and Worm pages.

Option 1: Enter the sequence(s) of RNAi reagent(s)

You need to select the species first and then enter RNAi sequence with ID.

For example:

  1.      GCGGCGGTTAACAATACCGAA
  2.      GCGGCGGTTAACAATACCGAA
  3.      GCGGCGGTTAACAATACCGAA
  4.      TTTTTGTTTGTTTTACTCCGA
  5.      CTGGTTATACCAGGACATTGA

The 1 st column is user-specificied RNAi reagent ID and the 2nd column is the gene-specific sequence of RNAi reagent.

Option 2: Enter the primer sequences for dsRNA reagent(s)

You need to select the species first and then enter primer-pair sequence with ID.

For example:

1      ATCTGCTGCTGCAGGTTC           GCCAACTATCAGTTCCCAAC
2      CTCATCATTTATAAGATGGTCAC      AAATGAAAAACAAGTTCAAACTG
3      TTTCTTTGTCCTCCTTATTCTTC      GAACCCGCCACCCTATGA
4      ATATGTGGGCTATAGCGAAAT        CAGAACACGGCCGACAC
5      GTTTTTTTTCCCCTCCATCTTT       GAGTGGGCAAAGTACAAGAT
6      GACGCTGCTTATCTTCCTGG         CTTGGTCTTCTTGCCAAAGC

The 1 st column is user-specified RNAi reagent ID.

The 2nd column is the gene-specific sequence of first PCR primer.

The 3rd column is the gene-specific sequence of second PCR primer.

Option 3: Enter RNAi reagent ID(s) from the following resources.

  • DRSC amplicon (DRSC amplicon ID)
  • DKFZ amplicon (BKN amplicon ID)
  • TRiP transgenic fly (stock ID or hairpin ID)
  • NIG transgenic fly (stock ID)
  • VDRC transgenic fly (transformant ID)

For example:

  • JF02592
  • DRSC32366
  • DRSC32367
  • HMS00794
  • JF01257
  • 30044R-1
  • BKN20541

Option 4: Enter gene identifier(s) to query the available fly RNAi reagents such as FlyBase gene identifier and gene symbol.

For example:

  • wg
  • s-cup
  • RpL27
  • FBgn0003996
  • CG10006

Option 5: Enter sequence(s) and query all the fly RNAi reagents that target these sequence(s)

For example:

>wg exon2

GGGCATTGCCAAGGTCGGCGAACCCAACAACATTACGCCCATCATGTACA

TGGACCCAGCGATCCACTCTACGTTGAGAAGGAAACAGCGACGCCTGGTC

AGGGACAATCCCGGTGTACTGGGAGCCCTGGTCAAGGGCGCCAACTTGGC

CATTAGCGAGTGCCAACACCAGTTCAGAAATCGCCGCTGGAAC

TGCTCGA

CGAGAAACTTCTCGAGGGGCAAAAATCTATTCGGCAAAATCGTTGATCGA

G

Publication to cite and for more information